Adrenal steroidogenesis disruption caused by HDL/cholesterol suppression in diethylstilbestrol-treated adult male rat
| Autor: | Michiko Sato, Satoko Haeno, Naoyuki Maeda, Aika Uto, Kousuke Yamaguchi, Hiroshi Yokota |
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| Rok vydání: | 2015 |
| Předmět: |
Male
0301 basic medicine Apolipoprotein E medicine.medical_specialty Very low-density lipoprotein Endocrinology Diabetes and Metabolism Cholesterol VLDL Diethylstilbestrol Biology Rats Sprague-Dawley 03 medical and health sciences chemistry.chemical_compound Apolipoproteins E Endocrinology Corticosterone Internal medicine Adrenal Glands medicine Animals Endocrine system Estrogens Non-Steroidal Apolipoprotein A-I Cholesterol Cholesterol HDL Cholesterol LDL Rats 030104 developmental biology Liver chemistry Toxicity Steroids lipids (amino acids peptides and proteins) Homeostasis Liver Circulation medicine.drug |
| Zdroj: | Endocrine. 52:148-156 |
| ISSN: | 1559-0100 1355-008X |
| DOI: | 10.1007/s12020-015-0732-5 |
| Popis: | The synthetic estrogen diethylstilbestrol is used to prevent miscarriages and as a therapeutic treatment for prostate cancer, but it has been reported to have adverse effects on endocrine homeostasis. However, the toxicity mechanism is poorly understood. Recently, we reported that diethylstilbestrol impairs adrenal steroidogenesis via cholesterol insufficiency in adult male rats. In the present study, we found that the adrenal cholesterol level was significantly reduced without of the decrease in other precursors in the adrenal steroidogenesis 24 h after a single dose of diethylstilbestrol (0.33 μg/g body mass). The serum HDL/cholesterol level was also reduced only 12 h after the diethylstilbestrol exposure. The level of Apo E, which is indispensable for HDL/cholesterol maturation, was decreased in both the HDL and VLDL/LDL fractions, whereas the level of Apo A1, which is an essential constituent of HDL, was not altered in the HDL fraction. Because the liver is a major source of Apo E and Apo A1, the secretion rates of these proteins were examined using a liver perfusion experiment. The secretion rate of Apo A1 from the liver was consistent between DES-treated and control rats, but that of Apo E was comparatively suppressed in the DES-treated rats. The disruption of adrenal steroidogenesis by diethylstilbestrol was caused by a decrease in serum HDL/cholesterol, which is the main source of adrenal steroidogenesis, due to the inhibition of Apo E secretion from the liver. |
| Databáze: | OpenAIRE |
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