IL-6 and PACAP Receptor Expression and Localization after Global Brain Ischemia in Mice
| Autor: | Sachiko Yofu, Yoshihiro Wada, Keisuke Nakamura, Masashi Tsuchida, Akira Yoshikawa, Satoru Arata, Tomoya Nakamachi, Nori Imai, Nobuyuki Kagami, Daisuke Tsuchikawa, Seiji Shioda, Motohide Hori |
|---|---|
| Rok vydání: | 2012 |
| Předmět: |
medicine.medical_specialty
Time Factors Receptors Vasoactive Intestinal Polypeptide Type I Receptor expression Ischemia DNA Single-Stranded Hippocampus Nerve Tissue Proteins Biology Real-Time Polymerase Chain Reaction Neuroprotection Brain Ischemia Brain ischemia Mice Cellular and Molecular Neuroscience Internal medicine medicine Animals Carotid Stenosis RNA Messenger CA1 Region Hippocampal Homeodomain Proteins Glial fibrillary acidic protein Interleukin-6 Pyramidal Cells General Medicine medicine.disease Endocrinology medicine.anatomical_structure Astrocytes Disease Progression biology.protein Receptors Vasoactive Intestinal Peptide Type II Microglia NeuN Receptors Pituitary Adenylate Cyclase-Activating Polypeptide Type I Astrocyte |
| Zdroj: | Journal of Molecular Neuroscience. 48:518-525 |
| ISSN: | 1559-1166 0895-8696 |
| DOI: | 10.1007/s12031-012-9819-0 |
| Popis: | Pituitary adenylate cyclase-activating polypeptide (PACAP) exerts a neuroprotective action against ischemic damage. This action is mediated by the interleukin-6 (IL-6) pathway. However, as the expression patterns of PACAP receptors and IL-6 following ischemia are not understood, we evaluated them in the mouse hippocampus in response to ischemia induced by bilateral common carotid artery occlusion. Real-time PCR determination of PAC1R mRNA expression in the hippocampus was significantly elevated on day 7 after ischemia. VPAC1R mRNA expression was significantly decreased 3 days after the ischemic episode, while VPAC2R mRNA expression showed a nonsignificant tendency to increase on day 7. IL-6 mRNA expression was significantly increased on day 3 and peaked on day 7 after ischemia. The mRNA expression of activity-dependent neuroprotective protein, which is a neuroprotective factor stimulated by PACAP, remained virtually unchanged in response to ischemia. IL-6 immunoreactivity was detected in the CA1 pyramidal cell layer and colocalized with the neuronal marker NeuN on day 1 after ischemia. On day 3, irregularly shaped IL-6-immunopositive cells colocalized with the astrocytic marker glial fibrillary acidic protein but not with the microglial marker Iba1. PAC1R immunoreactivity co-labeled with IL-6 immunoreactivity. These results suggest that PACAP could stimulate IL-6 secretion by neurons during the acute phase after an ischemic episode and thereafter by astrocytes during the subacute phase. |
| Databáze: | OpenAIRE |
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