Insights into an alternative pathway for glycerol metabolism in a glycerol kinase deficient Pseudomonas putida KT2440
| Autor: | William Casey, Shane T. Kenny, Kevin O’ Connor, Hendrik Ballerstedt, Nick Wierckx, Lars M. Blank, Tanja Narancic, Meg Walsh |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: |
0303 health sciences
Glycerol kinase biology Fatty acid metabolism 030306 microbiology Mutant Wild type Dehydrogenase biology.organism_classification Pseudomonas putida 03 medical and health sciences chemistry.chemical_compound chemistry Biochemistry Glyceraldehyde Glycerol 030304 developmental biology |
| Zdroj: | bioRxiv |
| DOI: | 10.1101/567230 |
| Popis: | Pseudomonas putidaKT2440 is known to metabolise glycerol via glycerol-3-phosphate using glycerol kinase an enzyme previously described as critical for glycerol metabolism (1). However, when glycerol kinase was knocked out inP. putidaKT2440 it retained the ability to use glycerol as the sole carbon source, albeit with a much-extended lag period and 2 fold lower final biomass compared to the wild type strain. A metabolomic study identified glycerate as a major and the most abundant intermediate in glycerol metabolism in this mutated strain with levels 21-fold higher than wild type. Erythrose-4-phosphate was detected in the mutant strain, but not in the wild type strain. Glyceraldehyde and glycraldehyde-3-phosphate were detected at similar levels in the mutant strain and the wild type. Transcriptomic studies identified 191 genes that were more than 2-fold upregulated in the mutant compared to the wild type and 175 that were down regulated. The genes involved in short chain length fatty acid metabolism were highly upregulated in the mutant strain. The genes encoding 3-hydroxybutyrate dehydrogenase were 5.8-fold upregulated and thus the gene was cloned, expressed and purified to reveal it can act on glyceraldehyde but not glycerol as a substrate. |
| Databáze: | OpenAIRE |
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