Stable Chromosome Condensation Revealed by Chromosome Conformation Capture
| Autor: | Tom A. Hartl, Roger D. Kornberg, Kyle P. Eagen |
|---|---|
| Rok vydání: | 2015 |
| Předmět: |
Euchromatin
Heterochromatin Chromosomal Puffs Biology General Biochemistry Genetics and Molecular Biology Article Chromosome conformation capture 03 medical and health sciences 0302 clinical medicine medicine Animals 030304 developmental biology Polytene Chromosomes Genetics Cell Nucleus 0303 health sciences Polytene chromosome Biochemistry Genetics and Molecular Biology(all) Diploidy Chromatin Cell biology Cell nucleus medicine.anatomical_structure Drosophila melanogaster Genetic Techniques Premature chromosome condensation Larva 030217 neurology & neurosurgery |
| Zdroj: | Cell. 163(4):934-946 |
| ISSN: | 0092-8674 |
| DOI: | 10.1016/j.cell.2015.10.026 |
| Popis: | SummaryChemical cross-linking and DNA sequencing have revealed regions of intra-chromosomal interaction, referred to as topologically associating domains (TADs), interspersed with regions of little or no interaction, in interphase nuclei. We find that TADs and the regions between them correspond with the bands and interbands of polytene chromosomes of Drosophila. We further establish the conservation of TADs between polytene and diploid cells of Drosophila. From direct measurements on light micrographs of polytene chromosomes, we then deduce the states of chromatin folding in the diploid cell nucleus. Two states of folding, fully extended fibers containing regulatory regions and promoters, and fibers condensed up to 10-fold containing coding regions of active genes, constitute the euchromatin of the nuclear interior. Chromatin fibers condensed up to 30-fold, containing coding regions of inactive genes, represent the heterochromatin of the nuclear periphery. A convergence of molecular analysis with direct observation thus reveals the architecture of interphase chromosomes. |
| Databáze: | OpenAIRE |
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