Zebrafish Model of Neuroblastoma Metastasis
| Autor: | Taylor M. Levee, Kok Siong Yeo, Cassie J. Howe, Shizhen Zhu, Zuag Paj Her |
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| Rok vydání: | 2021 |
| Předmět: |
General Immunology and Microbiology
General Chemical Engineering General Neuroscience Transgene Cancer Biology medicine.disease biology.organism_classification Article General Biochemistry Genetics and Molecular Biology Metastasis Animals Genetically Modified Disease Models Animal Neuroblastoma Genome editing In vivo Live cell imaging medicine Cancer research Animals Zebrafish |
| Zdroj: | J Vis Exp |
| ISSN: | 1940-087X |
| Popis: | Zebrafish has emerged as an important animal model to study human diseases, especially cancer. Along with the robust transgenic and genome editing technologies applied in zebrafish modeling, the ease of maintenance, high-yield productivity, and powerful live imaging altogether make the zebrafish a valuable model system to study metastasis and cellular and molecular bases underlying this process in vivo. The first zebrafish neuroblastoma (NB) model of metastasis was developed by overexpressing two oncogenes, MYCN and LMO1, under control of the dopamine-beta-hydroxylase (dβh) promoter. Co-overexpressed MYCN and LMO1 led to the reduced latency and increased penetrance of neuroblastomagenesis, as well as accelerated distant metastasis of tumor cells. This new model reliably reiterates many key features of human metastatic NB, including involvement of clinically relevant and metastasis-associated genetic alterations; natural and spontaneous development of metastasis in vivo; and conserved sites of metastases. Therefore, the zebrafish model possesses unique advantages to dissect the complex process of tumor metastasis in vivo. |
| Databáze: | OpenAIRE |
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