Pharyngeal pouches provide a niche microenvironment for arch artery progenitor specification

Autor: Yu Cao, Qiang Wang, Linwei Li, Guozhu Ning, Aihua Mao, Mingming Zhang, Jie Liu
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Mesoderm
Pharyngeal pouch
Morphogenesis
Biology
Pharyngeal arch artery morphogenesis
Progenitor specification
03 medical and health sciences
Dorsal aorta
0302 clinical medicine
Cell Movement
BMP signal
medicine
Animals
Cell Lineage
Progenitor cell
Molecular Biology
Zebrafish
030304 developmental biology
Progenitor
Body Patterning
0303 health sciences
Stem Cells
Embryogenesis
Endothelial Cells
Gene Expression Regulation
Developmental
Cell Differentiation
Arteries
biochemical phenomena
metabolism
and nutrition
Zebrafish Proteins
Stem Cells and Regeneration
Cell biology
medicine.anatomical_structure
Branchial Region
Cellular Microenvironment
Bone Morphogenetic Proteins
Ectopic expression
Niche microenvironment
030217 neurology & neurosurgery
Pharyngeal arch
Developmental Biology
Signal Transduction
Zdroj: Development (Cambridge, England)
article-version (VoR) Version of Record
ISSN: 1477-9129
0950-1991
Popis: The paired pharyngeal arch arteries (PAAs) are transient blood vessels connecting the heart with the dorsal aorta during embryogenesis. Although PAA malformations often occur along with pharyngeal pouch defects, the functional interaction between these adjacent tissues remains largely unclear. Here, we report that pharyngeal pouches are essential for PAA progenitor specification in zebrafish embryos. We reveal that the segmentation of pharyngeal pouches coincides spatiotemporally with the emergence of PAA progenitor clusters. These pouches physically associate with pharyngeal mesoderm in discrete regions and provide a niche microenvironment for PAA progenitor commitment by expressing BMP proteins. Specifically, pouch-derived BMP2a and BMP5 are the primary niche cues responsible for activating the BMP/Smad pathway in pharyngeal mesoderm, thereby promoting progenitor specification. In addition, BMP2a and BMP5 play an inductive function in the expression of the cloche gene npas4l in PAA progenitors. cloche mutants exhibit a striking failure to specify PAA progenitors and display ectopic expression of head muscle markers in the pharyngeal mesoderm. Therefore, our results support a crucial role for pharyngeal pouches in establishing a progenitor niche for PAA morphogenesis via BMP2a/5 expression.
Summary: Pharyngeal pouch-derived BMP2a and BMP5 are the key niche cues that induce arch artery progenitor specification during zebrafish embryonic development.
Databáze: OpenAIRE
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