Composition of lipid A in the S and R forms of Shigella dysenteriae serovar 1
| Autor: | Č. Michalec, Karel Stránský, Tomáš Trnka, Jack Levin, Jaroslav Julák, Jiří Šourek |
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| Rok vydání: | 1992 |
| Předmět: |
Shigella dysenteriae
Lipopolysaccharide Immunology Carbohydrates Microbiology Lethal Dose 50 Lipid A Mice chemistry.chemical_compound Animals Serotyping Sugar Limulus Test Glucosamine Mice Inbred ICR biology Fatty Acids biology.organism_classification Enterobacteriaceae Aminosugar chemistry Biochemistry Limulus amebocyte lysate lipids (amino acids peptides and proteins) Bacteria |
| Zdroj: | Zentralblatt für Bakteriologie. 277:429-435 |
| ISSN: | 0934-8840 |
| DOI: | 10.1016/s0934-8840(11)80467-6 |
| Popis: | A chemical analysis was performed of lipid A, isolated by acid hydrolysis of the lipopolysaccharide from the S and R forms of Shigella dysenteriae serovar 1. Differences in the moiety of both lipids and sugars were compared. The lipid portion consisted of a homologous series of fatty acids ranging from C12:0 to C18:0 (predominant homologues, C12:0, C14:0 and C16:0) and the homologous series of 3-hydroxy acids ranging from C12:0 to C16:0 (predominant homologue, 3-OH-C14:0). The major sugar portion consisted of D-glucosamine. The toxicity of lipid A in mice (LD50) ranged between 300-400 micrograms/mouse, and values from the Limulus amebocyte lysate assay were recorded as titres of 10(-5) to 10(-6) mg/ml. |
| Databáze: | OpenAIRE |
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