Mycobactericidal activity of human natural, monoclonal, and recombinant yeast killer toxin-like antibodies
| Autor: | M. Gerloni, Walter Magliani, Antonio Cassone, Stefania Conti, Antonella Salati, D Bertolotti, Luciano Polonelli, F. Fanti |
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| Předmět: |
Idiotype
Pichia anomala medicine.drug_class Lymphocyte Antitubercular Agents Receptors Cell Surface Monoclonal antibody Pichia Microbiology law.invention law medicine Immunology and Allergy Candida albicans biology Antibodies Monoclonal Drug Resistance Microbial Mycobacterium tuberculosis Mycotoxins biology.organism_classification Drug Resistance Multiple Killer Factors Yeast Recombinant Proteins Antibodies Anti-Idiotypic Infectious Diseases medicine.anatomical_structure Monoclonal Recombinant DNA biology.protein Antibody |
| Zdroj: | ResearcherID |
| Popis: | Human natural (KTAb), murine monoclonal (KTMAb), and single-chain recombinant (KTScFv) candidacidal antibodies representing the internal image of a killer toxin from the yeast Pichia anomala (KT), characterized by a wide spectrum of antibiotic activity, exerted a lethal effect against a KT-sensitive multidrug-resistant isolate of Mycobacterium tuberculosis. KTMAb and KTScFv were produced by the hybridoma and DNA technologies, respectively, from the spleen lymphocytes of animals immunized with the idiotype of a KT-neutralizing MAb (MAb KT4), while KTAb were purified against MAb KT4 from the vaginal fluid of women infected with Candida albicans cells bearing an idiotype-like KT cell wall receptor. Mycobactericidal activity was related to the binding of KTAb, KTMAb, and KTScFv to the cell surface of KT-sensitive bacterial cells and was prevented by specific absorption of KT-like antibodies onto MAb KT4. These data identify a novel potentially useful immunotherapeutic approach to tuberculosis. |
| Databáze: | OpenAIRE |
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