Reduction in formation of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP)-induced aberrant crypt foci in the rat colon by docosahexaenoic acid (DHA)
| Autor: | Mitsuharu Masuda, Atsuko Oguri, Kazunori Fukuda, Mami Takahashi, Takashi Sugimura, Kazunaga Yazawa, Yukari Totsuka, Keiji Wakabayashi |
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| Rok vydání: | 1997 |
| Předmět: |
Male
Cancer Research medicine.medical_specialty Docosahexaenoic Acids Colon chemistry.chemical_compound DNA Adducts Internal medicine medicine Animals Anticarcinogen Carcinogen chemistry.chemical_classification 2-Amino-1-methyl-6-phenylimidazo(4 5-b)pyridine Azoxymethane Imidazoles food and beverages General Medicine Neoplasms Experimental Fish oil digestive system diseases Rats Inbred F344 Rats Endocrinology chemistry Biochemistry Docosahexaenoic acid Heterocyclic amine Colonic Neoplasms Carcinogens Precancerous Conditions Aberrant crypt foci |
| Zdroj: | Carcinogenesis. 18(10) |
| ISSN: | 0143-3334 |
| Popis: | Docosahexaenoic acid (DHA), a major component of fish oil, suppresses the formation and growth of aberrant crypt foci induced by 1,2-dimethylhydrazine and azoxymethane. In the present study we examined the effects of intragastric gavage administration of DHA on the yield of rat colonic aberrant crypt foci due to treatment with a heterocyclic amine, 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PhIP), which induces colon cancer in male F344 rats and is considered to be a possible human colon carcinogen. Male F344 rats were given a standard diet (AIN-76A) and received 10 doses of PhIP (75 mg/kg body wt, by intragastric intubation, on days 1-5 and 8-12) with or without intragastric application of 1 ml DHA 4 h prior to each carcinogen treatment, followed by further DHA dosing. The numbers of PhIP-induced aberrant crypt foci per colon after 4 and 12 weeks DHA administration were significantly reduced to 47 and 38% respectively of the values obtained when PhIP alone was used. The mean number of aberrant crypts per focus was also decreased by DHA treatment. At week 4 the PhIP-DNA adduct levels in the colon of rats from the PhIP+DHA group were approximately two thirds of the PhIP group value. The results thus suggest that DHA exerts a preventive effect on PhIP-induced colon carcinogenesis. |
| Databáze: | OpenAIRE |
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