Disaccharide Residues are Required for Native Antifreeze Glycoprotein Activity
| Autor: | Huib J. Bakker, Manfred Wagner, Giulia Giubertoni, Konrad Meister, Arthur L. DeVries, Yuling Sun, Jie Liu, David Y. W. Ng |
|---|---|
| Rok vydání: | 2021 |
| Předmět: |
Circular dichroism
Magnetic Resonance Spectroscopy Recrystallization (geology) Polymers and Plastics Stereochemistry Disaccharide Bioengineering 02 engineering and technology Disaccharides 010402 general chemistry 01 natural sciences Article Biomaterials Hydrophobic effect chemistry.chemical_compound Antifreeze Proteins Materials Chemistry Threonine Glycoproteins Alanine chemistry.chemical_classification Ice Hydrogen Bonding 021001 nanoscience & nanotechnology 0104 chemical sciences chemistry Antifreeze 0210 nano-technology Glycoprotein |
| Zdroj: | Biomacromolecules |
| ISSN: | 1526-4602 1525-7797 |
| DOI: | 10.1021/acs.biomac.1c00313 |
| Popis: | Antifreeze glycoproteins (AFGPs) are able to bind to ice, halt its growth, and are the most potent inhibitors of ice recrystallization known. The structural basis for AFGP’s unique properties remains largely elusive. Here we determined the antifreeze activities of AFGP variants that we constructed by chemically modifying the hydroxyl groups of the disaccharide of natural AFGPs. Using nuclear magnetic resonance, two-dimensional infrared spectroscopy, and circular dichroism, the expected modifications were confirmed as well as their effect on AFGPs solution structure. We find that the presence of all the hydroxyls on the disaccharides is a requirement for the native AFGP hysteresis as well as the maximal inhibition of ice recrystallization. The saccharide hydroxyls are apparently as important as the acetyl group on the galactosamine, the α-linkage between the disaccharide and threonine, and the methyl groups on the threonine and alanine. We conclude that the use of hydrogen-bonding through the hydroxyl groups of the disaccharide and hydrophobic interactions through the polypeptide backbone are equally important in promoting the antifreeze activities observed in the native AFGPs. These important criteria should be considered when designing synthetic mimics. |
| Databáze: | OpenAIRE |
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