Primary and quaternary structure of the catabolic ornithine carbamoyltransferase from Pseudomonas aeruginosa. Extensive sequence homology with the anabolic ornithine carbamoyltransferases of Escherichia coli
| Autor: | Paul Falmagne, Heinz Baur, Ernst E. Luethi, Victor Stalon, D Haas |
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| Rok vydání: | 1987 |
| Předmět: |
Macromolecular Substances
Biology medicine.disease_cause Biochemistry chemistry.chemical_compound Ornithine Carbamoyltransferase Sequence Homology Nucleic Acid Escherichia coli medicine Amino Acid Sequence Amino Acids Cloning Molecular Gene Peptide sequence chemistry.chemical_classification Base Sequence Nucleic acid sequence Ornithine Biological Evolution Amino acid Genes chemistry Pseudomonas aeruginosa Transfer RNA Electrophoresis Polyacrylamide Gel |
| Zdroj: | European Journal of Biochemistry. 166:111-117 |
| ISSN: | 1432-1033 0014-2956 |
| DOI: | 10.1111/j.1432-1033.1987.tb13489.x |
| Popis: | We have determined the complete nucleotide sequence of the arcB gene from Pseudomonas aeruginosa strain PAO and we have purified the arcB product, the catabolic ornithine carbamoyltransferase (EC 2.1.3.3), to apparent homogeneity from the same strain. The N-terminal amino acid sequence, the total amino acid composition and the subunit size of the purified enzyme were in agreement with nucleotide sequencing results, which predict a polypeptide of 336 amino acids (Mr 38,108). Crosslinking experiments suggest that the native enzyme (apparent Mr approx. 420,000) basically consists of a trimer aggregating to form nonamers or dodecamers. The arcB gene of P. aeruginosa had strong homology with the argF and argI genes which code for the anabolic ornithine carbamoyltransferase isoenzymes in Escherichia coli; 63% of the nucleotides and 57% of the amino acids were absolutely conserved in arcB and argF. This indicates a close evolutionary relationship between these genes although their products have different physiological functions in the cell. Under conditions of induction (energy depletion) the catabolic ornithine carbamoyltransferase represented greater than or equal to 10% of the total cellular protein. Like other highly expressed Pseudomonas genes, the arcB gene was found not to use seven codons which correspond to minor or weakly interacting tRNA species in E. coli. |
| Databáze: | OpenAIRE |
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