Proteome analysis of acute kidney injury – Discovery of new predominantly renal candidates for biomarker of kidney disease
| Autor: | Bianca Kiers, Rafael Dariolli, Karina Helena Morais Cardozo, Alexandre C. Pereira, Kallyandra Padilha, Valdemir Melechco Carvalho, Pamella Araujo Malagrino, José Eduardo Krieger, Tamiris Carneiro Gois, Adriana C. C. Girardi, Gabriela Venturini, Jéssica Silva Salgueiro, Silvia M. Titan, Patrícia Schneider Yogi |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Pathology medicine.medical_specialty Kidney Cortex Proteome Swine Renal cortex 030232 urology & nephrology Biophysics Biochemistry Diabetic nephropathy 03 medical and health sciences 0302 clinical medicine Intensive care Animals Medicine Kidney Renal ischemia business.industry Acute kidney injury Proteins Adherens Junctions Acute Kidney Injury medicine.disease 030104 developmental biology medicine.anatomical_structure Gene Expression Regulation Reperfusion Injury Biomarker (medicine) business Biomarkers Transcription Factors Kidney disease |
| Zdroj: | Journal of Proteomics. 151:66-73 |
| ISSN: | 1874-3919 |
| Popis: | The main bottleneck in studies aiming to identify novel biomarkers in acute kidney injury (AKI) has been the identification of markers that are organ and process specific. Here, we have used different tissues from a controlled porcine renal ischemia/reperfusion (I/R) model to identify new, predominantly renal biomarker candidates for kidney disease. Urine and serum samples were analyzed in pre-ischemia, ischemia (60 min) and 4, 11 and 16 h post-reperfusion, and renal cortex samples after 24 h of reperfusion. Peptides were analyzed on the Q-Exactive™. In renal cortex proteome, we observed an increase in the synthesis of proteins in the ischemic kidney compared to the contralateral, highlighted by transcription factors and epithelial adherens junction proteins. Intersecting the set of proteins up- or down-regulated in the ischemic tissue with both serum and urine proteomes, we identified 6 proteins in the serum that may provide a set of targets for kidney injury. Additionally, we identified 49, being 4 predominantly renal, proteins in urine. As prove of concept, we validated one of the identified biomarkers, dipeptidyl peptidase IV, in a set of patients with diabetic nephropathy. In conclusion, we identified 55 systemic proteins, some of them predominantly renal, candidates for biomarkers of renal disease. Biological significance The main bottleneck in studies aiming to identify novel biomarkers in acute kidney injury (AKI) has been the identification of markers that are predominantly renal. In fact, putative biomarkers for this condition have also been identified in a number of other clinical scenarios, such as cardiovascular diseases, chronic kidney failure or in patients being treated in intensive care units from a number of conditions. Here we propose a comprehensive, sequential screening procedure able to identify and validate potential biomarkers for kidney disease, using kidney ischemia/reperfusion as a paradigm for a kidney pathological event. |
| Databáze: | OpenAIRE |
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