Antimicrobial Action of Hydrolyzed Chitosan against Spoilage Yeasts and Lactic Acid Bacteria of Fermented Vegetables
| Autor: | Carole Beaulieu, Tony Savard, Claude P. Champagne, Isabelle Boucher |
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| Rok vydání: | 2002 |
| Předmět: |
Colony Count
Microbial Saccharomyces bayanus Chitin macromolecular substances Microbiology Hydrolysate Saccharomyces chemistry.chemical_compound Anti-Infective Agents Vegetables Food science Chitosanase Chitosan biology technology industry and agriculture biology.organism_classification Yeast Anti-Bacterial Agents Culture Media Lactic acid Molecular Weight carbohydrates (lipids) Lactobacillus Microscopy Electron Biochemistry chemistry Fermentation Bacteria Lactic acid fermentation Food Science |
| Zdroj: | Journal of Food Protection. 65:828-833 |
| ISSN: | 0362-028X |
| DOI: | 10.4315/0362-028x-65.5.828 |
| Popis: | The antimicrobial properties of various chitosan-lactate polymers (ranging from 0.5 to 1.2 MDa in molecular weight) against two yeasts isolated from fermented vegetables and against three lactic acid bacteria from a mixed starter for sauerkraut on methylene blue agar (MBA) and in vegetable juice medium (VJM) were investigated. Chitosan-lactate reduced the growth of all microorganisms in solid (MBA) as well as in liquid (VJM) medium. In MBA, a concentration of 5 g/liter was needed to inhibit the growth of Saccharomyces bayanus, while I g/liter was sufficient to inhibit the growth of Saccharomyces unisporus. Lactic acid bacteria were also inhibited in this range of concentrations. The low-molecular-weight chitosan-lactate DP3 (0.5 kDa) was most efficient in solid medium (MBA), and inhibitory activities decreased with increasing hydrolysate lengths, In liquid medium (VJM), 0.5 g of chitosan-lactate per liter reduced the growth rates for both yeasts, but 10 g/liter was insufficient to prevent yeast growth. Intermediate-molecular-weight chitosan-lactate (5 kDa) was more efficient than chitosan of low molecular weight. Native chitosan (1.2 MDa) showed no inhibition in either medium. Microscopic examination of S. unisporus Y-42 after treatment with chitosan-lactate DP25 showed agglutination of a refractive substance on the entire cell wall, suggesting an interaction between chitosan and the cell wall. When chitosanase was added to the culture media containing chitosan-lactate, refractive substances could not be observed. Cette etude evalue les proprietes antifongiques et antibacteriennes de polymeres lactate-chitosane hydrolyse et natif contre 2 levures d'alteration et 3 bacteries lactiques utilisees dans la fermentation lactique de legumes. De plus, l'interaction entre le chitosane et les cellules microbiennes est etudiee au niveau microscopique. Les resultats montrent que les activites antimicrobiennes des polymeres sont influencees par le degre d'hydrolyse du chitosane, le microorganisme cible et la composition du milieu. |
| Databáze: | OpenAIRE |
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