Identification of an argpyrimidine-modified protein in human red blood cells from schizophrenic patients: A possible biomarker for diseases involving carbonyl stress
| Autor: | Shin Koike, Mitsuhiro Miyashita, Masanari Itokawa, Shoichi Nishimoto, Toshihiro Suzuki, Yosuke Kibune, Tasuku Kayama, Yuki Ogasawara, Yasue Horiuchi, Makoto Arai, Yo-ichi Ishida |
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| Rok vydání: | 2017 |
| Předmět: |
Male
Ornithine 0301 basic medicine Erythrocytes Arginine Biophysics Selenium-Binding Proteins Risk Assessment Sensitivity and Specificity Biochemistry Protein Carbonylation 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Japan Prevalence medicine Humans Selenium binding Molecular Biology biology Methylglyoxal Reproducibility of Results Cell Biology medicine.disease Molecular biology Red blood cell Pyrimidines 030104 developmental biology medicine.anatomical_structure chemistry Schizophrenia biology.protein Biomarker (medicine) Advanced glycation end-product Female Antibody Biomarkers 030217 neurology & neurosurgery Kidney disease |
| Zdroj: | Biochemical and Biophysical Research Communications. 493:573-577 |
| ISSN: | 0006-291X |
| DOI: | 10.1016/j.bbrc.2017.08.150 |
| Popis: | Argpyrimidine (ARP) is an advanced glycation end product thought to be generated from a reaction between methylglyoxal and arginine residues in proteins. In this study, we observed marked accumulation of an approximately 56 kD protein, reactive to anti-ARP antibodies, in the red blood cells (RBCs) of some patients with refractory schizophrenia. This ARP-modified protein was purified from the blood of schizophrenic patients and identified as selenium binding protein 1 (SBP1) by LC-MS/MS. This is the first report of ARP-modified proteins accumulating in RBCs of patients with diseases involving carbonyl stress. We also observed high accumulation of ARP-modified SBP1 in the RBCs of patients with chronic kidney disease. Therefore, this modified protein may be a novel marker of carbonyl stress. |
| Databáze: | OpenAIRE |
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