p16, cyclin D1, Ki-67, and AMACR as markers for dysplasia in Barrett esophagus
| Autor: | Xue Ying Shi, Anthony S.-Y. Leong, Brahm Bhagwandeen |
|---|---|
| Rok vydání: | 2008 |
| Předmět: |
Adult
Male Pathology medicine.medical_specialty Histology Esophageal Neoplasms Racemases and Epimerases Adenocarcinoma Gastroenterology Pathology and Forensic Medicine Barrett Esophagus Cyclin D1 CDKN2A Internal medicine Biomarkers Tumor Medicine Humans Esophagus Cyclin-Dependent Kinase Inhibitor p16 Aged Aged 80 and over biology business.industry Middle Aged medicine.disease Immunohistochemistry Staining Neoplasm Proteins Medical Laboratory Technology medicine.anatomical_structure Ki-67 Antigen Dysplasia Ki-67 biology.protein business Immunostaining |
| Zdroj: | Applied immunohistochemistrymolecular morphology : AIMM. 16(5) |
| ISSN: | 1533-4058 |
| Popis: | Barrett esophagus (BE) is an established precursor of esophageal adenocarcinoma (AdenoCa). One hundred and one cases of BE diagnosed by esophageal biopsy and resections were examined morphologically for dysplasia. These were categorized as BE without dysplasia (n=25), indefinite for dysplasia (IND, n=17), low-grade dysplasia (LGD, n=18), high-grade dysplasia (HGD, n=15), and AdenoCa (n=26). Immunostaining for p16 (INK4A/CDKN2A), Cyclin D1 (CCND1), Ki-67, and alpha-methylacyl-CoA racemase (AMACR) was employed to assess their potential as diagnostic discriminators. Abnormal p16 expression (negative, cytoplasmic, or combined cytoplasmic and nuclear staining) was present in all categories, rising from 68% in BE without dysplasia to 100% in AdenoCa, with cytoplasmic staining only showing a significant correlation with the severity of dysplasia. Cyclin D1 expression was present in almost all cases, but high expression (>50% cells positive) was displayed mostly in HGD and AdenoCa (46.7% and 42.3%, respectively). Ki-67 index increased with the severity of dysplasia and labeling extended from the lower third of the crypts to the superficial epithelium. The frequency of AMACR-positivity was 12% in BE, 47.1% in IND, 44.4% in LGD, 93.3% in HGD, and 96.2% in AdenoCa. The intensity and extent of AMACR staining also increased with the severity of dysplasia. Aberrant p16 and high-cyclin D1 expression may reflect early genetic events during the progression of Barrett-associated carcinogenesis. Cytoplasmic staining of p16 is specific. It may represent a different pathway of p16 dysfunction. The pattern and extent of Ki-67 staining and AMACR overexpression are useful additional parameters for identifying dysplasia in BE. |
| Databáze: | OpenAIRE |
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