Pathogenesis of avian pneumovirus infection in turkeys
| Autor: | Kakambi V. Nagaraja, Daniel P. Shaw, Sally Noll, Faris F. Jirjis, David A. Halvorson |
|---|---|
| Rok vydání: | 2002 |
| Předmět: |
0301 basic medicine
Mycoplasma gallisepticum Male Pathology medicine.medical_specialty Turkeys 040301 veterinary sciences Minnesota 030106 microbiology Fluorescent Antibody Technique Enzyme-Linked Immunosorbent Assay Mycoplasma synoviae Antibodies Viral Newcastle disease Virus 0403 veterinary science 03 medical and health sciences Cytopathogenic Effect Viral Chlorocebus aethiops medicine Animals Vero Cells Ornithobacterium rhinotracheale Poultry Diseases Bordetella avium Paramyxoviridae Infections General Veterinary biology Histocytochemistry Reverse Transcriptase Polymerase Chain Reaction Mycoplasma meleagridis 04 agricultural and veterinary sciences biology.organism_classification medicine.disease Avian pneumovirus Nasal Mucosa DNA Viral Female Metapneumovirus |
| Zdroj: | Veterinary pathology. 39(3) |
| ISSN: | 0300-9858 |
| Popis: | Avian pneumovirus (APV) is the cause of a respiratory disease of turkeys characterized by coughing, ocular and nasal discharge, and swelling of the infraorbital sinuses. Sixty turkey poults were reared in isolation conditions. At 3 weeks of age, serum samples were collected and determined to be free of antibodies against APV, avian influenza, hemorrhagic enteritis, Newcastle disease, Mycoplasma gallisepticum, Mycoplasma synoviae, Mycoplasma meleagridis, Ornithobacterium rhinotracheale, and Bordetella avium. When the poults were 4 weeks old, they were inoculated with cell culture–propagated APV (APV/Minnesota/turkey/2a/97) via the conjunctival spaces and nostrils. After inoculation, four poults were euthanatized every 2 days for 14 days, and blood, swabs, and tissues were collected. Clinical signs consisting of nasal discharge, swelling of the infraorbital sinuses, and frothy ocular discharge were evident by 2 days postinoculation (PI) and persisted until day 12 PI. Mild inflammation of the mucosa of the nasal turbinates and infraorbital sinuses was present between days 2 and 10 PI. Mild inflammatory changes were seen in tracheas of poults euthanatized between days 4 and 10 PI. Antibody to APV was detected by day 7 PI. The virus was detected in tissue preparations and swabs of nasal turbinates and infraorbital sinuses by reverse transcription polymerase chain reaction, virus isolation, and immunohistochemical staining methods between days 2 and 10 PI. Virus was detected in tracheal tissue and swabs between days 2 and 6 PI using the same methods. In this experiment, turkey poults inoculated with tissue culture-propagated APV developed clinical signs similar to those seen in field cases associated with infection with this virus. |
| Databáze: | OpenAIRE |
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