Relative expression of genes associated with adhesion to bovine mammary epithelial cells by Streptococcus uberis
| Autor: | Claudia Raspanti, Silvana Andrea Dieser, Alumine Soledad Fessia, Maria Sol Renna, Liliana Odierno |
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| Rok vydání: | 2020 |
| Předmět: |
040301 veterinary sciences
media_common.quotation_subject Gene Expression STREPTOCOCCUS UBERIS Bacterial Adhesion Cell Line BOVINE MASTITIS Ciencias Biológicas 0403 veterinary science 03 medical and health sciences Mammary Glands Animal ADHERENCE Biología Celular Microbiología Streptococcal Infections Animals RELATIVE EXPRESSION Internalization Mastitis Bovine Gene 030304 developmental biology media_common Streptococcus uberis 0303 health sciences General Veterinary biology Biofilm Streptococcus Epithelial Cells 04 agricultural and veterinary sciences Adhesion biology.organism_classification Molecular biology Genes Bacterial Biofilms INTERNALIZATION Cattle Female BIOFILM CIENCIAS NATURALES Y EXACTAS |
| Zdroj: | Research in Veterinary Science. 132:33-41 |
| ISSN: | 0034-5288 |
| Popis: | Streptococcus uberis is one of the most prevalent environmental pathogens of bovine mastitis. Biofilm growth ability by S. uberis looks to depend first upon the adherence of cells to a surface. The S. uberis ability to adhere to mammary gland epithelia might provide an advantage to colonize the lactating mammary gland. The objectives of this study were (a) to select S.uberis strains according to their ability to form biofilm, (b) to determine adherence to and internalization into MAC-T cells and (c) to investigate the expression profile adherence genes in these S. uberis strains. For the assays, the MAC-T bovine mammary epithelial cell line was used. Relative expression of genes acdA, lmb, scpA, sua, fbp and lbp was quantified by RT-qPCR. We observed that the RC38 strain from clinical bovine mastitis showed in the six genes higher values than control in both conditions. While the strain with greater ability to adhere, from clinical mastitis and biofilm producer (RC29) evidenced higher values in group 1 (G1) (bacteria after the initial contact with MAC-T cells) and decrease in group 2 (G2) (both adhered and internalized bacteria) than control. Strains with a moderate or strong capacity for biofilm production showed significantly lower relative expression values in the G2. In all adherence associated genes, strain RC19 showed relative expression values incremented in G1, while in G2 decreased expression. In conclusion, we did not find a single profile of relative expression because the relative expression levels of each gene differed depending on the strain and the co-culture stage of S. uberis cells from which RNA was obtained. Fil: Fessia, Alumine Soledad. Universidad Nacional de Río Cuarto. Facultad de Ciencias Exactas, Fisicoquímicas y Naturales. Departamento de Microbiología e Inmunología; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Dieser, Silvana Andrea. Universidad Nacional de Río Cuarto; Argentina. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina Fil: Renna, Maria Sol. Consejo Nacional de Investigaciones Científicas y Técnicas. Centro Científico Tecnológico Conicet - Santa Fe. Instituto de Ciencias Veterinarias del Litoral. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias. Instituto de Ciencias Veterinarias del Litoral; Argentina Fil: Raspanti, Claudia Gabriela. Universidad Nacional del Litoral. Facultad de Ciencias Veterinarias; Argentina Fil: Odierno, Liliana Mónica. Consejo Nacional de Investigaciones Científicas y Técnicas; Argentina |
| Databáze: | OpenAIRE |
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