Translocation of cytosolic annexin 2 to a Triton-insoluble membrane subdomain upon nicotine stimulation of chromaffin cultured cells
| Autor: | Francoise Regnouf, Jean-Pierre Henry, Isabelle Sagot, Louise-Anne Pradel |
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| Jazyk: | angličtina |
| Předmět: |
Nicotine
Octoxynol Chromaffin Cells Detergents Biophysics Annexin Biology Endocytosis Biochemistry Exocytosis Cytosol Glucosides Structural Biology Caveolin Genetics Animals Phosphorylation Cytoskeleton Molecular Biology Integral membrane protein Annexin A2 Cells Cultured Chromaffin cell Cell Membrane Membrane Proteins Biological Transport Cell Biology Cell biology Membrane protein Cattle |
| Zdroj: | FEBS Letters. (2-3):229-234 |
| ISSN: | 0014-5793 |
| DOI: | 10.1016/S0014-5793(97)00594-2 |
| Popis: | To gain a better understanding of the function of annexin 2, we have investigated the subcellular distribution of the monomeric and heterotetrameric forms of annexin 2 and their relationship to the cytoskeleton upon stimulation of chromaffin cells. Quantitative immunoblotting has revealed that in resting cells a large amount of annexin 2 is monomeric and cytosolic. Upon nicotine stimulation 80% of total annexin 2 becomes associated with a Triton-X100-insoluble fraction where the monomeric and the heterotetrameric forms are found. The translocation of monomeric annexin 2 is Ca2+-dependent and complete at 1 μM free Ca2+. We have shown that about 66% of the annexin 2 associated with the Triton-X100-insoluble fraction is soluble in octylglucoside while the remnants are insoluble in the detergent and remain likely associated with actin filaments and associated cytoskeleton proteins. The octylglucoside-soluble fraction contains integral proteins from the plasma membrane and from granule membrane, but does not contain caveolin. Moreover, upon nicotine stimulation, a redistribution of proteins was detected in this fraction. These dynamic processes appear concomitantly with the phosphorylation of annexin 2 in this compartment and with catecholamine release. It is suggested that the soluble octylglucoside fraction may represent a special lipidic membrane compartment where the NSF attachment proteins and the cytosolic proteins like annexin 2 and rab3a may become concentrated upon stimulation of the cell. The presence of annexin 2 is consistent with its proposed function on granule and target membrane proteins required for the close apposition of two distinct membranes and supports its functional role in the regulated exocytosis/endocytosis process. |
| Databáze: | OpenAIRE |
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