Angiogenic Effect of Leptin in the Quail Chorioallantoic Membrane
| Autor: | Michal Zeman, Ľubor Košťál, Pavel Výboh, Boris Bilčík, Božena Šárniková |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
Leptin
fractal dimension medicine.medical_specialty animal structures lcsh:Veterinary medicine General Veterinary Angiogenesis Embryogenesis digestive oral and skin physiology chorioallantoic membrane Embryo Stimulation Biology In ovo Quail Chorioallantoic membrane Endocrinology Internal medicine biology.animal embryonic structures medicine japanese quail lcsh:SF600-1100 |
| Zdroj: | Acta Veterinaria Brno, Vol 79, Iss 1, Pp 13-17 (2010) |
| ISSN: | 1801-7576 0001-7213 |
| Popis: | Leptin, the product ofobgene, beside its key role in the control of body weight and food consumption, can be involved in the control of embryonic development. Leptin administrationin ovoaccelerated the embryonic and post-embryonic development in Japanese quail. Although the mechanisms of leptin effects on growth and development acceleration are not clear, stimulation of angiogenesis represents one of plausible explanations. Therefore, the aim of the present study was to investigate the pro-angiogenic effect of leptinin vivoin the quail chorioallantoic membrane (CAM). The recombinant murine leptin (10, 100, and 1000 ng) was applied eitherex ovoon the CAM surface ofex ovoincubated embryos at embryonic day 7 (ED7) orin ovointo the egg albumen at ED5. Changes in blood vessels were quantified by the fractal analysis providing the fractal dimension (Df) estimate. Leptin administeredin ovowas more efficient in stimulation of angiogenesis than theex ovotreatment, since 10 ng dose elicited significantly higher (P< 0.001) stimulation of vessel development of the CAM under the air cell than it did afterex ovocultivation. Our study confirmed that exogenously applied leptin was able to stimulate angiogenesis in CAM. Leptin-mediated stimulation of angiogenesis may improve nutrient utilization from the yolk and explain at least partially the accelerating effect of leptin on avian embryo growth and development. |
| Databáze: | OpenAIRE |
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