Proliferation and differentiation markers of colorectal adenocarcinoma and their correlation with clinicopathological factors

Autor: Miloš Diklić, Gordana Jovčić, Slobodan Todorovic, Tijana Subotički, Bojana B. Beleslin-Cokic, Olivera Mitrović Ajtić, Vladan P. Čokić
Rok vydání: 2016
Předmět:
Vascular Endothelial Growth Factor A
Pathology
medicine.medical_specialty
cyclin B1
Adenocarcinoma
Metastasis
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Carcinoembryonic antigen
Biomarkers
Tumor
Humans
Medicine
030212 general & internal medicine
Cyclin B1
Adenocarcinoma of colon
E-cadherin
mCEA
cyclin B1
erythropoietin receptor
vascular endothelial growth factor
erythropoietin
Lymph node
Cell Proliferation
mCEA
Adenocarcinoma of colon
biology
vascular endothelial growth factor
business.industry
E-cadherin
Cell Differentiation
General Medicine
medicine.disease
Antigens
Differentiation
3. Good health
Erythropoietin receptor
Vascular endothelial growth factor
medicine.anatomical_structure
chemistry
030220 oncology & carcinogenesis
biology.protein
Immunohistochemistry
erythropoietin
Colorectal Neoplasms
business
erythropoietin receptor
Zdroj: Turkish Journal of Medical Sciences
Volume: 46, Issue: 4 1168-1176
ISSN: 1300-0144
1303-6165
Popis: Background/aim: The purpose of this study was to investigate proliferation and differentiation markers in colorectal adenocarcinoma and their correlation with clinicopathological factors. Materials and methods: Samples were collected from 38 patients with colorectal adenocarcinoma and 10 healthy controls. E-cadherin, carcinoembryonic antigen (mCEA), cyclin B1, vascular endothelial growth factor (VEGF), and erythropoietin (EPO) receptor (EPOR) were examined by immunohistochemistry; VEGF and EPO were examined by real-time PCR. Results: The tumor samples were mostly characterized by large dimension (pT3), moderate level of differentiation (G2), negative lymph node status (N0), and no metastasis. Cyclin B1 and VEGF gene and protein expressions were significantly higher in tumor tissues than in control tissues; E-cadherin expression was significantly decreased in tumor samples and in positive correlation with mCEA. EPO was almost undetectable in tumor tissues of colorectal adenocarcinoma. Significant positive correlation was detected between tumor size and cyclin B1, tumor grade, and lymph node status. Conclusion: Decreased expression of EPO, high levels of VEGF and cyclin B1 expression, predominant moderate tumor differentiation, absence of metastasis, and negative lymph node status may suggest low level of aggressiveness, better prognosis, and longer patient survival.
Databáze: OpenAIRE
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