Uptake, hydrolysis, and metabolism of pyridoxine-5'-beta-D-glucoside in Caco-2 cells
| Autor: | Robert J. McMahon, Amy Mackey, Jesse F. Gregory, Justin H. Townsend |
|---|---|
| Rok vydání: | 2004 |
| Předmět: |
Pyridoxal
medicine.medical_treatment Medicine (miscellaneous) Lactose Hydrolysis chemistry.chemical_compound Glucosides Enzymatic hydrolysis parasitic diseases Hydrolase medicine Humans Lactase Nutrition and Dietetics Sodium Pyridoxine B vitamins Kinetics chemistry Biochemistry Pyridoxamine Caco-2 Cells medicine.drug |
| Zdroj: | The Journal of nutrition. 134(4) |
| ISSN: | 0022-3166 |
| Popis: | An important dietary source of vitamin B-6, pyridoxine-5'-beta-D-glucoside (PNG), exhibits only partial bioavailability, which is limited by the extent of enzymatic cleavage of the beta-glucosidic bond to release metabolically available pyridoxine (PN). This laboratory showed that the intestinal hydrolysis of PNG is catalyzed by cytosolic PNG hydrolase (PNGH) and brush border lactase-phlorizin hydrolase (LPH). LPH-catalyzed PNG hydrolysis in vitro is competitively inhibited by lactose. In the present study, the uptake and hydrolysis of PNG were examined in Caco-2 human colon carcinoma cells, which express a functional LPH but exhibit no PNGH activity. PNG uptake at 37 degrees C was linear over 5-500 micromol/L PNG. Uptake was not significantly reduced when Na(+) was substituted with K(+), Li(+), or Tris in the medium. Increasing PNG concentration in the medium did not change intracellular concentrations of PN, pyridoxamine (PM), pyridoxamine 5'-phosphate (PMP), or pyridoxal 5'-phosphate (PLP); however, intracellular pyridoxal (PL) concentration increased. Intracellular PNG concentration was not significantly reduced in the presence of lactose, but the concentration of PL declined in proportion to extracellular lactose (P = 0.01). These results indicate that PNG can be absorbed intact in a Na(+)-independent process and is taken up by passive diffusion. The presence of lactose in this in vitro model of intestinal uptake reduced the enzymatic hydrolysis of PNG by lactase. |
| Databáze: | OpenAIRE |
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