Optimized analysis of intracellular adenosine and guanosine phosphates in Escherichia coli
| Autor: | Peter Neubauer, Sylke Meyer, Matthias Reuss, Naruemol Noisommit-Rizzi |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Guanine
Biophysics medicine.disease_cause Biochemistry chemistry.chemical_compound Adenine nucleotide medicine Bioreactor Escherichia coli Nucleotide Molecular Biology Chromatography High Pressure Liquid chemistry.chemical_classification Chromatography Adenine Nucleotides Cell Biology Adenosine Guanine Nucleotides chemistry Evaluation Studies as Topic Energy Metabolism Quantitative analysis (chemistry) Intracellular medicine.drug |
| Zdroj: | Analytical biochemistry. 271(1) |
| ISSN: | 0003-2697 |
| Popis: | To investigate the intracellular concentrations of adenosine phosphates in Escherichia coli, especially during bioreactor cultivations, a method that enables reproducible determination of adenosine phosphates in culture solutions containing at least 0.25 g dry cell weight/L has been developed. The detection limits of AMP, ADP, and ATP were found to be as low as 1 pmol. The method involves fast sampling, instantaneous inactivation of cell metabolism, extraction of nucleotides, and quantitative analysis by ion-pair reversed-phase HPLC. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|
Full Text from ScienceDirect