A single centrifugation method for isolating fat droplets from cells and tissues
| Autor: | Trevor M. Shew, Nathan E. Wolins, Lydia-Ann L.S. Harris, James R. Skinner |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2012 |
| Předmět: |
Male
lipid droplets Adipose tissue Centrifugation fatty acid/metabolism Cell Separation QD415-436 Biology Cell Fractionation liver Biochemistry lipids Mice Endocrinology Cytosol Lipid droplet Cell Line Tumor Organelle Adipocytes Methods Animals Chromatography brown adipose tissue Cell Biology Fasting Rats adipose tissue Membrane Solubility Cellular component Female Cell fractionation |
| Zdroj: | Journal of Lipid Research, Vol 53, Iss 5, Pp 1021-1025 (2012) |
| ISSN: | 0022-2275 |
| Popis: | Fat droplets (FDs) have important roles in cellular energy regulation. Isolating FDs from either cells or tissue continues to be important for studying these organelles. Here, we describe a procedure wherein whole homogenates of cultured cells or tissue are fractionated with a single centrifugation step in a standard microcentrifuge. This procedure reproducibly yields three fractions highly enriched in either FDs, soluble cellular components, or sedimentable organelles/membranes. |
| Databáze: | OpenAIRE |
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