Antioxidant properties, antimicrobial and anti-adhesive activities of DCS1 lipopeptides from Bacillus methylotrophicus DCS1
| Autor: | Noomen Hmidet, Moncef Nasri, Nawel Jemil, Hanen Ben Ayed, Angeles Manresa |
|---|---|
| Přispěvatelé: | Universitat de Barcelona |
| Rok vydání: | 2017 |
| Předmět: |
0106 biological sciences
0301 basic medicine Microbiology (medical) Antioxidant DPPH Linoleic acid medicine.medical_treatment 030106 microbiology Lipopeptides like substances lcsh:QR1-502 Bacillus Biology Gram-Positive Bacteria 01 natural sciences Microbiology Bacterial Adhesion lcsh:Microbiology Antioxidants Lipid peroxidation Lipopeptides 03 medical and health sciences chemistry.chemical_compound Anti-Infective Agents 010608 biotechnology Gram-Negative Bacteria Spectroscopy Fourier Transform Infrared Anti-adhesive activities medicine Biofilm Bacils Bacillus methylotrophicus DCS1 Antimicrobial Thin-layer chromatography In vitro Bacillus (Bacteria) chemistry Biochemistry Biofilms Chromatography Thin Layer Lipid Peroxidation Research Article |
| Zdroj: | Recercat. Dipósit de la Recerca de Catalunya instname Dipòsit Digital de la UB Universidad de Barcelona BMC Microbiology, Vol 17, Iss 1, Pp 1-11 (2017) BMC Microbiology |
| ISSN: | 1471-2180 |
| DOI: | 10.1186/s12866-017-1050-2 |
| Popis: | Background The present work aims to investigate the antioxidant and antimicrobial activities as well as the potential of DCS1 lipopeptides produced by Bacillus methylotrophicus DCS1 strain at inhibition and disruption of biofilm formation. Results The produced biosurfactants were characterized as lipopeptides molecules by using thin layer chromatography (TLC) and Fourier transform infrared spectroscopy (FT-IR). The DCS1 lipopeptides were assayed for their antioxidant activity through five different tests. The scavenging effect on DPPH radicals at a concentration of 1 mg mL−1 was 80.6%. The reducing power reached a maximum value of 3.0 (OD700 nm) at 2 mg mL−1. Moreover, the DCS1 lipopeptides exhibited a strong inhibition of β-carotene bleaching by linoleic acid assay with 80.8% at 1 mg mL−1 and showed good chelating ability and lipid peroxidation inhibition. The in vitro antimicrobial activity of DCS1 lipopeptides showed that they display significant antibacterial and antifungal activities. The anti-adhesive activity of DCS1 lipopeptides was evaluated against several pathogenic microorganisms. The lipopeptides showed excellent anti-adhesive activity, even at low concentrations, in a polystyrene surface pre-treatment against all the microorganisms tested. Further, they can disrupt performed biofilms. Conclusion This study shows the potentiality of DCS1 lipopeptides as natural antioxidants, antimicrobial and/or anti-adhesive agent for several biomedical and industrial applications. |
| Databáze: | OpenAIRE |
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