CD8+ T cells as a source of IFN-γ production in human cutaneous leishmaniasis
| Autor: | Tahereh Shahrestani, Hossein Keshavarz, Rosita Edalat, Fereidoun Mahboudi, Ali Khamesipour, Mahmoud Nateghi Rostami, Abdolfattah Sarrafnejad |
|---|---|
| Přispěvatelé: | Medical Parasitology and Mycology Department, School of public health, The University of Hong Kong (HKU)-The University of Hong Kong (HKU)-Tehran University of Medical Sciences, Faculty of Health, Qom University of Medical Sciences, Biotechnology Research Center, Institut Pasteur d'Iran, Réseau International des Instituts Pasteur (RIIP)-Réseau International des Instituts Pasteur (RIIP), Immunology Department, Center for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, The study was funded by School of Public Health, Center for Research & Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Iran, and National Science Foundation of Iran. |
| Jazyk: | angličtina |
| Rok vydání: | 2010 |
| Předmět: |
CD4-Positive T-Lymphocytes
Male MESH: Interleukin-13 MESH: Interleukin-10 MESH: Human Experimentation CD8-Positive T-Lymphocytes Interleukin 21 0302 clinical medicine MESH: Leishmania major Cytotoxic T cell Leishmania major IFN-c Production Cells Cultured 0303 health sciences Interleukin-13 lcsh:Public aspects of medicine MESH: CD4-Positive T-Lymphocytes CD8+ T Cells MESH: CD8-Positive T-Lymphocytes 3. Good health Interleukin-10 Infectious Diseases [SDV.IMM]Life Sciences [q-bio]/Immunology Female Research Article MESH: Cells Cultured Adult Human Cutaneous Leishmaniasis lcsh:Arctic medicine. Tropical medicine lcsh:RC955-962 MESH: Interferon-gamma 030231 tropical medicine Leishmaniasis Cutaneous Antigens Protozoan Biology 03 medical and health sciences Interferon-gamma MESH: Gene Expression Profiling Immune system Antigen Cutaneous leishmaniasis medicine Humans RNA Messenger 030304 developmental biology MESH: RNA Messenger MESH: Humans Gene Expression Profiling Public Health Environmental and Occupational Health lcsh:RA1-1270 MESH: Adult biology.organism_classification medicine.disease Leishmania MESH: Interleukin-5 MESH: Leishmaniasis Cutaneous Molecular biology MESH: Male Human Experimentation Immunology Immunology/Immune Response Interleukin-5 MESH: Female CD8 MESH: Antigens Protozoan |
| Zdroj: | PLoS Neglected Tropical Diseases PLoS Neglected Tropical Diseases, Public Library of Science, 2010, 4 (10), pp.e845. ⟨10.1371/journal.pntd.0000845⟩ PLoS Neglected Tropical Diseases, Vol 4, Iss 10, p e845 (2010) |
| ISSN: | 1935-2727 1935-2735 |
| Popis: | Background In human leishmaniasis Th1/Th2 dichotomy similar to murine model is not clearly defined and surrogate marker(s) of protection is not yet known. In this study, Th1/Th2 cytokines (IL-5, IL-10, IL-13 and IFN-γ) profile induced by purified CD4+/CD8+ T cells in response to Leishmania antigens were assessed at transcript and protein levels in 14 volunteers with a history of self-healing cutaneous leishmaniasis (HCL) and compared with 18 healthy control volunteers. Methodology/Principal Findings CD4+/CD8+/CD14+ cells were purified from peripheral blood using magnetic beads; CD4+/CD8+ T cells were co-cultured with autologous CD14+ monocytes in the presence of soluble Leishmania antigens (SLA). Stimulation of either CD4+ T cells or CD8+ T cells of HCL volunteers with SLA induced a significantly (P Author Summary Cutaneous leishmaniasis (CL) is usually a self-healing skin lesion caused by different species of Leishmania parasite. Resistance and susceptibility of mice to Leishmania major infection is associated with two types of CD4+ T lymphocytes development: Th1 type response with production of cytokine IFN-γ is associated with resistance, whereas Th2 type response with production of cytokines IL-4 and IL-5 is associated with susceptibility. A clear Th1/Th2 dichotomy similar to murine model is not defined in human leishmaniasis and we need as much information as possible to define marker(s) of protection. We purified CD4+/CD8+ T cells, stimulated them with Leishmania antigens and analysed gene and protein expression of Th1/Th2 cytokines in volunteers with a history of self-healing CL who are presumed to be protected against further Leishmania infection. We have seen significant upregulation of IFN-γ gene expression and high IFN-γ production in the Leishmania stimulated CD4+ T cells and CD8+ T cells. We concluded that both antigen-specific IFN-γ producing CD4+ Th1 cells and IFN-γ producing CD8+ T cells contribute to the long term protection in individuals with a history of CL. This proves the importance of CD8+ T cells as a source of IFN-γ in Th1-like immune responses. |
| Databáze: | OpenAIRE |
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