Separation of three glucocorticoid-binding fractions from cytosol of rat heart
| Autor: | Sergei M. Danilov, Yu.M. Seleznev, Vladimir N. Smirnov |
|---|---|
| Rok vydání: | 1979 |
| Předmět: |
Receptors
Steroid Fraction (chemistry) Biochemistry Binding Competitive Dexamethasone chemistry.chemical_compound Endocrinology Cytosol Receptors Glucocorticoid Transcortin Corticosterone medicine Animals Testosterone Chromatography biology Binding protein Myocardium Rats chemistry Liver Ionic strength biology.protein Chromatography Gel Carrier Proteins Glucocorticoid medicine.drug Homogenization (biology) |
| Zdroj: | Journal of steroid biochemistry. 10(2) |
| ISSN: | 0022-4731 |
| Popis: | By chromatography on AcA-34 and AcA-44 ultra-gels three glucocorticoid-binding fractions were separated from heart cytosol preparations of adrenalectomized rats. In several respects the glucocorticoid binding factor of the first fraction resembles glucocorticoid cytoreceptor of liver. At low ionic strength this binding protein is eluted in 7.1 S form whereas at high ionic strength it is dissociated into 5.2 S form. The second fraction contains transcortin, which can be washed out of the tissue by a prolonged perfusion. It apparently contaminates cytosol during tissue homogenization. The third fraction (like transcortin) binds corticosterone but does not bind dexamethasone. It differs from transcortin in Chromatographic behaviour and in sensitivity to SH-blocking agents. The three glucocorticoid-binding fractions described are also found in liver cytosol. |
| Databáze: | OpenAIRE |
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