An automated microfluidic platform for C. elegans embryo arraying, phenotyping, and long-term live imaging
| Autor: | Johan Auwerx, Shreya Narasimhan, Matteo Cornaglia, Laurent Mouchiroud, Martin A. M. Gijs, Thomas Lehnert, Alexis Marette, Virginija Jovaisaite |
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| Rok vydání: | 2014 |
| Předmět: |
Embryo
Nonmammalian Population ved/biology.organism_classification_rank.species Microfluidics Morphogenesis Embryonic Development 02 engineering and technology Biology Article 03 medical and health sciences Automation Live cell imaging Mitochondrial unfolded protein response Animals education Model organism Caenorhabditis elegans 030304 developmental biology 0303 health sciences education.field_of_study Multidisciplinary ved/biology Optical Imaging Embryo 021001 nanoscience & nanotechnology biology.organism_classification Embryonic stem cell Cell biology Mitochondria Phenotype Unfolded Protein Response 0210 nano-technology |
| Zdroj: | Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Studies of the real-time dynamics of embryonic development require a gentle embryo handling method, the possibility of long-term live imaging during the complete embryogenesis, as well as of parallelization providing a population’s statistics, while keeping single embryo resolution. We describe an automated approach that fully accomplishes these requirements for embryos of Caenorhabditis elegans, one of the most employed model organisms in biomedical research. We developed a microfluidic platform which makes use of pure passive hydrodynamics to run on-chip worm cultures, from which we obtain synchronized embryo populations, and to immobilize these embryos in incubator microarrays for long-term high-resolution optical imaging. We successfully employ our platform to investigate morphogenesis and mitochondrial biogenesis during the full embryonic development and elucidate the role of the mitochondrial unfolded protein response (UPRmt) within C. elegans embryogenesis. Our method can be generally used for protein expression and developmental studies at the embryonic level, but can also provide clues to understand the aging process and age-related diseases in particular. |
| Databáze: | OpenAIRE |
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