Evaluation of Cytokine Messenger RNA Expression in Peripheral Blood Mononuclear Cells from Dogs with Canine Demodicosis
| Autor: | Kenji Tani, Tadayoshi Nomura, Yasuho Taura, Hisashi Inokuma, Satoshi Une, Takafumi Ohnishi, Munekazu Nakaichi, Toshiharu Hayashi, Masahiro Morimoto, Sanae Hayashiya |
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| Rok vydání: | 2002 |
| Předmět: |
Male
Mite Infestations medicine.medical_specialty medicine.medical_treatment Biology Peripheral blood mononuclear cell Statistics Nonparametric Dogs Interferon Internal medicine medicine Demodicosis Animals Dog Diseases RNA Messenger Skin Diseases Parasitic Messenger RNA General Veterinary Reverse Transcriptase Polymerase Chain Reaction Interleukin medicine.disease Cytokine Endocrinology Real-time polymerase chain reaction Immunology Leukocytes Mononuclear Cytokines Female Tumor necrosis factor alpha medicine.drug |
| Zdroj: | Journal of Veterinary Medical Science. 64:513-518 |
| ISSN: | 1347-7439 0916-7250 |
| DOI: | 10.1292/jvms.64.513 |
| Popis: | Using RT-PCR and semi-quantitative PCR, mRNA expression for canine interferon (IFN)-gamma, interleukin (IL)-2, IL-4, IL-5, IL-10, tumor necrosis factor (TNF)-alpha and transforming growth factor (TGF)-beta in peripheral blood mononuclear cells (PBMCs) was examined in dogs with or without demodicosis. mRNA expression for IFN-gamma as well as TNF-alpha in dogs with demodicosis (localized (LD) and generalized (GD)) was slightly lower than those in dogs without demodicosis (healthy controls). Expression of IL-5 mRNA in dogs with demodicosis was higher than that in control dogs, but there were no significant differences in IL-4 and IL-10 mRNA expression levels among the three groups. On the other hand, expression levels of TGF-beta mRNA in dogs with GD were higher than those in control dogs and dogs with LD. The expression levels of IL-5 and TGF-beta mRNA decreased in all three dogs with GD which showed resolution of the clinical signs. Taken together, these results suggest that the Th2-like response in PBMCs from dogs with demodicosis is up-regulated, and that subsequent increased expression of IL-5 and TGF-beta mRNA in dogs with GD is reversible after treatment. Therefore, these cytokines, particularly IL-5, might be a useful clinical index of the clinical course in demodicosis. Also, increased TGF-beta mRNA expression might be a key factor for revealing the difference in the mechanism of onset between LD and GD. |
| Databáze: | OpenAIRE |
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