Immunochemical studies on gastric and intestinal digestion of soybean glycinin and beta-conglycinin in vivo
| Autor: | René Toullec, E. N. C. Mills, Laurence Quillien, Didier Levieux, M. R. A. Morgan, Jean-Paul Lallès, P. Salgado, HM Tukur |
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| Přispěvatelé: | Laboratoire du jeune ruminant, Institut National de la Recherche Agronomique (INRA), UR 0724 Unité de recherche Biochimie et technologie des protéines, Institut National de la Recherche Agronomique (INRA)-Transformation des Produits Végétaux (T.P.V.)-Unité de recherche Biochimie et technologie des protéines (NANT LBTP), Station de recherches sur la viande, ProdInra, Migration |
| Jazyk: | angličtina |
| Rok vydání: | 1999 |
| Předmět: |
Meal
food.ingredient VIDANGE STOMACALE food and beverages General Chemistry Biology [SDV.IDA] Life Sciences [q-bio]/Food engineering Small intestine 3. Good health Blot food medicine.anatomical_structure Biochemistry Plant protein TEST ELISA Skimmed milk [SDV.IDA]Life Sciences [q-bio]/Food engineering medicine Soybean Proteins General Agricultural and Biological Sciences Digestion Polyacrylamide gel electrophoresis |
| Zdroj: | Journal of Agricultural and Food Chemistry Journal of Agricultural and Food Chemistry, American Chemical Society, 1999, 47 (7), pp.2797-2806 |
| ISSN: | 0021-8561 1520-5118 |
| Popis: | Two experiments were conducted to study gastric and small intestinal digestion of soybean glycinin and [bêta]-conglycinin in preruminant calves fed milk replacers containing a mixture of skim milk powder and antigenic heated soybean flour. In experiment 1, duodenal passage of immunoreactive [bêta]-conglycinin lasted for a much longer time after the morning meal than that of glycinin. Western blotting revealed the early abomasal outflow of glycinin subunits that associated nearly intact basic polypeptides to partially degraded acidic polypeptides. Intact [bêta]-conglycinin was evidenced at most sampling times. In experiment 2, intact basic glycinin (Mr = 21000) associated with partially digested acidic glycinin (7000 < Mr < 25000) was demonstrated in ileal digesta up to 8-10 h after the meal. [bêta]-Conglycinin immunoreactivity could not be evidenced by Western blotting in ileal digesta. |
| Databáze: | OpenAIRE |
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