Carbohydrate determination in honey samples by ion chromatography-mass spectrometry (HPAEC-MS)

Autor: Gabriele Capodaglio, Paolo Fontana, Raffaello Tedesco, Elena Barbaro, R. Zangrando, Andrea Gambaro, Annapaola Rizzoli, Valeria Malagnini
Jazyk: angličtina
Rok vydání: 2020
Předmět:
Anions
Mono- and oligosaccharide profiles
Italian honey
Ion chromatography
Carbohydrates
02 engineering and technology
Mass spectrometry
01 natural sciences
Biochemistry
Mass Spectrometry
Analytical Chemistry
chemistry.chemical_compound
Limit of Detection
and oligosaccharide profiles
Monosaccharide
Tetrasaccharide
Settore CHIM/01 - Chimica Analitica
Settore CHIM/10 - CHIMICA DEGLI ALIMENTI
Chromatography
High Pressure Liquid
High-performance anion-exchange chromatography-mass spectrometry (HPAEC-MS)
chemistry.chemical_classification
High-performance anion-exchange chromatography-mass spectrometry (HPAEC-MS) . Italian honey . Mono- and oligosaccharide profiles
Chromatography
High-performance anion-exchange chromatography–mass spectrometry (HPAEC-MS)
010401 analytical chemistry
digestive
oral
and skin physiology
fungi
Reproducibility of Results
food and beverages
Fructose
Repeatability
Honey
Oligosaccharide
Carbohydrate
021001 nanoscience & nanotechnology
Chromatography
Ion Exchange
0104 chemical sciences
chemistry
behavior and behavior mechanisms
Mono
0210 nano-technology
Zdroj: Analytical & bioanalytical chemistry
(2020). doi:10.1007/s00216-020-02732-3
info:cnr-pdr/source/autori:Tedesco, Raffaello; Barbaro, Elena; Zangrando, Roberta; Rizzoli, Annapaola; Malagnini, Valeria; Gambaro, Andrea; Fontana, Paolo; Capodaglio, Gabriele/titolo:Carbohydrate determination in honey samples by ion chromatography-mass spectrometry (HPAEC-MS)/doi:10.1007%2Fs00216-020-02732-3/rivista:Analytical & bioanalytical chemistry (Print)/anno:2020/pagina_da:/pagina_a:/intervallo_pagine:/volume
DOI: 10.1007/s00216-020-02732-3
Popis: Honey is a complex mixture of carbohydrates, in which the monosaccharides glucose and fructose are the most abundant compounds. Currently, more than 20 oligosaccharides have been identified in different varieties of honey normally at quite low concentration. A method was developed and validated using high-performance anion-exchange chromatography coupled to a mass spectrometry detector to investigate the composition of carbohydrates in honey samples. The method was tested for linearity range, trueness, instrumental and method detection and quantification limits, repeatability, and reproducibility. It was applied to determine seven monosaccharides, eight disaccharides, four trisaccharides, and one tetrasaccharide in various honey samples. The present work describes the composition of sugars in unifloral, multifloral, and some honeydew honey, which were produced and collected by beekeepers in the Trentino Alto-Adige region. Statistical techniques have been used to establish a relationship based on levels of carbohydrates among different Italian honey. The results emphasize that mono- and oligosaccharide profiles can be useful to discriminate different honeys according to their floral characteristics and inter-annual variability.
Databáze: OpenAIRE
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