Genetic Polymorphisms in Cytokine Genes in Colombian Patients with Ocular Toxoplasmosis
| Autor: | Juan Carlos Sepúlveda-Arias, Alejandra de-la-Torre, Rima McLeod, Carlos Naranjo-Galvis, Jorge Enrique Gómez-Marín, C. López de Mesa, Ney Alliey-Rodriguez, Luz Eliana Mantilla-Muriel, L. Beltrán-Angarita, Ian J. Begeman, X. Elcoroaristizabal-Martín |
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| Rok vydání: | 2018 |
| Předmět: |
Male
single nucleotide 0301 basic medicine Dna extraction Ocular toxoplasmosis Monocyte ocular Gene regulatory networks Disease predisposition Interleukin 10 0302 clinical medicine Gene Frequency Interleukin 1alpha Haplotype Gene Regulatory Networks Promoter Regions Genetic Priority journal Allele education.field_of_study biology Interleukin 1beta Gene regulatory network Promoter region Colombian Infectious Diseases Cytokines Female Disease Susceptibility Toxoplasmosis Human Adult Genotype Disease susceptibility Tumor necrosis factor Immunology Population Case control study Genetic predisposition to disease Gene interaction Single-nucleotide polymorphism Major clinical study Case-control studies Colombia Polymorphism Single Nucleotide Microbiology Article Ribonuclease 03 medical and health sciences Genetic susceptibility Genetics medicine Humans Genetic Predisposition to Disease Polymorphism Toxoplasmosis Ocular education Cytokine Allele frequency Gamma interferon Alleles Genetic risk Genetic polymorphism Genetic predisposition Promoter regions Toxoplasma gondii Single nucleotide polymorphisms Odds ratio biology.organism_classification medicine.disease Gene frequency Single nucleotide polymorphism 030104 developmental biology Haplotypes Susceptibility Case-Control Studies Genetic association 030221 ophthalmology & optometry Parasitology Fungal and Parasitic Infections genetic Controlled study |
| Zdroj: | Repositorio EdocUR-U. Rosario Universidad del Rosario instacron:Universidad del Rosario |
| ISSN: | 1098-5522 0019-9567 |
| DOI: | 10.1128/iai.00597-17 |
| Popis: | Toxoplasmosis is caused by infection with the protozoan parasite Toxoplasma gondii , which has the capacity to infect all warm-blooded animals worldwide. Toxoplasmosis is a major cause of visual defects in the Colombian population; however, the association between genetic polymorphisms in cytokine genes and susceptibility to ocular toxoplasmosis has not been studied in this population. This work evaluates the associations between polymorphisms in genes coding for the cytokines tumor necrosis factor alpha (TNF-α) (rs1799964, rs1800629, rs1799724, rs1800630, and rs361525), interleukin 1β (IL-1β) (rs16944, rs1143634, and rs1143627), IL-1α (rs1800587), gamma interferon (IFN-γ) (rs2430561), and IL-10 (rs1800896 and rs1800871) and the presence of ocular toxoplasmosis (OT) in a sample of a Colombian population (61 patients with OT and 116 healthy controls). Genotyping was performed with the “dideoxynucleotide (ddNTP) primer extension” technique. Functional-effect predictions of single nucleotide polymorphisms (SNPs) were done by using FuncPred. A polymorphism in the IL-10 gene promoter (−1082G/A) was significantly more prevalent in OT patients than in controls ( P = 1.93e−08; odds ratio [OR] = 5.27e+03; 95% confidence interval [CI] = 3.18 to 8.739; Bonferroni correction [BONF] = 3.48e−07). In contrast, haplotype “AG” of the IL-10 gene promoter polymorphisms (rs1800896 and rs1800871) was present at a lower frequency in OT patients ( P = 7e−04; OR = 0.10; 95% CI = 0.03 to 0.35). The +874A/T polymorphism of IFN-γ was associated with OT ( P = 3.37e−05; OR = 4.2; 95% CI = 2.478 to 7.12; BONF = 6.07e−04). Haplotype “GAG” of the IL-1β gene promoter polymorphisms (rs1143634, rs1143627, and rs16944) appeared to be significantly associated with OT ( P = 0.0494). The IL-10, IFN-γ, and IL-1β polymorphisms influence the development of OT in the Colombian population. |
| Databáze: | OpenAIRE |
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