Accurate and rapid identification of bacterial species from positive blood cultures with a DNA-based microarray platform: an observational study
| Autor: | Eveliina Tarkka, Heli Piiparinen, Minna Mäki, Sanna Laakso, C Carder, Laura E. Savolainen, Päivi Tissari, Sointu Mero, Alimuddin Zumla, Martti Vaara, Anne Aittakorpi, Vanya Gant, Merja Lindfors, Jim F. Huggett |
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| Rok vydání: | 2010 |
| Předmět: |
DNA Topoisomerase IV
DNA Bacterial Bacteremia Drug resistance Biology Polymerase Chain Reaction Sensitivity and Specificity law.invention Bacterial genetics Microbiology Sepsis 03 medical and health sciences 0302 clinical medicine Bacterial Proteins law Drug Resistance Bacterial medicine Humans Penicillin-Binding Proteins Blood culture 030212 general & internal medicine Polymerase chain reaction Oligonucleotide Array Sequence Analysis Bacteriological Techniques 0303 health sciences Bacteria medicine.diagnostic_test 030306 microbiology General Medicine Nucleic acid amplification technique medicine.disease 3. Good health Real-time polymerase chain reaction DNA Gyrase Genes Bacterial DNA microarray Nucleic Acid Amplification Techniques |
| Zdroj: | The Lancet. 375:224-230 |
| ISSN: | 0140-6736 |
| DOI: | 10.1016/s0140-6736(09)61569-5 |
| Popis: | Summary Background New DNA-based microarray platforms enable rapid detection and species identification of many pathogens, including bacteria. We assessed the sensitivity, specificity, and turnaround time of a new molecular sepsis assay. Methods 2107 positive blood-culture samples of 3318 blood samples from patients with clinically suspected sepsis were investigated for bacterial species by both conventional culture and Prove-it sepsis assay (Mobidiag, Helsinki, Finland) in two centres (UK and Finland). The assay is a novel PCR and microarray method that is based on amplification and detection of gyrB, parE , and mecA genes of 50 bacterial species. Operators of the test assay were not aware of culture results. We calculated sensitivity, specificity, and turnaround time according to Clinical and Laboratory Standards Institute recommendations. Findings 1807 of 2107 (86%) positive blood-culture samples included a pathogen covered by the assay. The assay had a clinical sensitivity of 94·7% (95% CI 93·6–95·7) and a specificity of 98·8% (98·1–99·2), and 100% for both measures for meticillin-resistant Staphylococcus aureus bacteraemia. The assay was a mean 18 h faster than was the conventional culture-based method, which takes an additional 1–2 working days. 34 of 3284 (1·0%) samples were excluded because of technical and operator errors. Interpretation Definitive identification of bacterial species with this microarray platform was highly sensitive, specific, and faster than was the gold-standard culture-based method. This assay could enable fast and earlier evidence-based management for clinical sepsis. Funding None. |
| Databáze: | OpenAIRE |
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