Melanogenesis effects of rice protein hydrolysate and its characteristic peptides Leu-Leu-Lys, Leu-Pro-Lys, and pyroGlu-Lys on UVB-induced human epidermal melanocyte cells
Autor: | Ruizeng Gu, Muyi Cai, Wang Jing, Ma Yong, Mingliang Li, Chen Liang, Ruixue Zhang, Wei Ying |
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Rok vydání: | 2020 |
Předmět: |
0301 basic medicine
Protein Hydrolysates Melanoma Experimental Peptide Melanocyte Real-Time Polymerase Chain Reaction Plant Proteins Dietary Hydrolysate Mass Spectrometry Melanin 03 medical and health sciences 0302 clinical medicine medicine Humans chemistry.chemical_classification Melanins Messenger RNA Monophenol Monooxygenase Hydrolysis Oryza General Medicine Microphthalmia-associated transcription factor Amino acid 030104 developmental biology medicine.anatomical_structure chemistry Biochemistry Rice protein 030220 oncology & carcinogenesis Melanocytes Peptides Reactive Oxygen Species Food Science Signal Transduction |
Zdroj: | Foodfunction. 11(10) |
ISSN: | 2042-650X |
Popis: | This study assessed the melanogenesis effects of rice protein hydrolysate (RPH) and explored the underlying molecular mechanism of its characteristic peptides. In this investigation, human epidermal melanocyte (PIG1) cells were used to establish a UVB-induced model to evaluate the effect of RPH on melanin content, tyrosinase activity, and reactive oxygen species (ROS) levels. High performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was employed to identify the peptide composition (2–4 amino acids) in RPH. Enzymatic hydrolysis was employed to screen the characteristic peptides Leu-Leu-Lys (LLK), Leu-Pro-Lys (LPK), and pyroGlu-Lys (pEK), while their effect on the molecular mechanism involved in the melanin synthesis process was further explored using quantitative real-time polymerase chain reaction (PCR) and western blotting. The results indicated that RPH reduced the melanin content, tyrosinase activity, and ROS production in PIG1 cells. The selected peptides LLK, LPK, and pEK from RPH reduced the expression of tyrosinase-related protein 1 (TRP-1) and tyrosinase-related protein 2 (TRP-2) and affected melanin synthesis by regulating the JNK/β-Trcp/NFκB-p65/MITF signaling pathway at the mRNA and protein levels. This study shows that RPH plays a vital role in the melanogenesis process, therefore, providing a theoretical basis for the use of RPH as a novel additive product. |
Databáze: | OpenAIRE |
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