Detailed characterization of Pinus ponderosa sporopollenin by infrared spectroscopy
| Autor: | Matt J. Kipper, Kevin J. Morey, June I. Medford, Alec Lutzke |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
0106 biological sciences
Spectrophotometry Infrared Infrared Phytochemicals Infrared spectroscopy Plant Science Horticulture medicine.disease_cause 01 natural sciences Biochemistry Biopolymers Sporopollenin Spectrophotometry Pollen medicine Molecule Molecular Biology Chemical decomposition chemistry.chemical_classification Molecular Structure medicine.diagnostic_test 010405 organic chemistry Chemistry General Medicine Polymer Carotenoids Pinus ponderosa 0104 chemical sciences Chemical engineering 010606 plant biology & botany |
| Zdroj: | Phytochemistry. 170:112195 |
| ISSN: | 0031-9422 |
| Popis: | In plant spores and pollen, sporopollenin occurs as a structural polymer with remarkable resistance to chemical degradation. This recalcitrant polymer is well-suited to analysis by non-destructive infrared spectroscopy. However, existing infrared characterization of sporopollenin has been limited in scope and occasionally contradictory. This study provides a comprehensive structural analysis of sporopollenin in the Pinus ponderosa pollen exine using infrared spectroscopy, including detailed band assignments, descriptions of chemical reactivity, and comparison to multiple reference substances. We observe that the infrared spectral characteristics of sporopollenin prepared by enzymatic digestion of the polysaccharide-based intine are largely consistent with a copolymer of aliphatic lipids and trans-4-hydroxycinnamic acid, without distinct contributions from α-pyrone or carotenoid substructures. |
| Databáze: | OpenAIRE |
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