A phytosterol-enriched saw palmetto supercritical CO2 extract ameliorates testosterone-induced benign prostatic hyperplasia by regulating the inflammatory and apoptotic proteins in a rat model

Autor: K Shyamprasad, K Venkatakrishna, Anitha, H V Sudeep, Ballal Amrutharaj
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Male
Cell death
medicine.medical_specialty
Urinary system
Urology
H&E stain
Prostatic Hyperplasia
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Western blot
Prostate
Saw palmetto
Serenoa
Medicine
Animals
Humans
Prostate enlargement
Testosterone
Rats
Wistar
030304 developmental biology
bcl-2-Associated X Protein
Inflammation
0303 health sciences
medicine.diagnostic_test
business.industry
Plant Extracts
Phytosterols
Chromatography
Supercritical Fluid
General Medicine
lcsh:Other systems of medicine
Hyperplasia
medicine.disease
lcsh:RZ201-999
Sitosterols
Rats
medicine.anatomical_structure
Complementary and alternative medicine
chemistry
Proto-Oncogene Proteins c-bcl-2
030220 oncology & carcinogenesis
Finasteride
Herbal medicine
business
Research Article
Phytotherapy
Zdroj: BMC Complementary and Alternative Medicine, Vol 19, Iss 1, Pp 1-10 (2019)
BMC Complementary and Alternative Medicine
ISSN: 1472-6882
DOI: 10.1186/s12906-019-2697-z
Popis: Background Benign prostatic hyperplasia (BPH) is a pathological condition affecting older men. BPH complications often lead to deterioration in the quality of life. Serenoa repens (Saw Palmetto) is used for treating lower urinary tract infections in traditional medicine. Methods This study was performed to compare the efficacy of β-sitosterol enriched saw palmetto oil (VISPO) and conventional saw palmetto oil (SPO) extracted using supercritical fluid extraction, in alleviating the BPH complications using testosterone-induced BPH model rats. The animals received testosterone (5 mg/kg s.c.) with or without SPO and VISPO (200 and 400 mg/kg b.w.) or Finasteride (1 mg/kg b.w.) p.o. for 28 days. At the end of the experiment, overnight fasted animals were euthanized, blood samples collected for serum analysis of testosterone. Prostate tissue histomorphology was examined by hematoxylin and eosin (H&E) staining. Western blot analysis was performed using prostate tissue homogenates. Results VISPO exhibited superior efficacy compared to SPO as evident from the significant decrease in prostate weight to body weight ratio, serum testosterone level and increase in growth inhibition of prostate tissue compared to BPH group (p Conclusion Our data provide experimental evidence that β-sitosterol enriched saw palmetto oil could be higher efficacious in treating the BPH complications compared to the conventional saw palmetto oil preparations.
Databáze: OpenAIRE
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