Frequency of hepatitis B surface antigen variants (HBsAg) in hepatitis B virus genotype B and C infected East- and Southeast Asian patients: Detection by the Elecsys ® HBsAg II assay
| Autor: | Bui Huu Hoang, Albert Kriegner, Wolfgang E. Kaminski, Cunling Yan, Pham Thi Thu Thuy, Min Xu, Zhen Zeng, Markus Nauck, Gaston Westergaard, Hyon Suk Kim, Stephan Pabinger, Kirsten Hübner, Yiwen Yan, Anja Seffner, Terry Wang, Yali Liu, Haohui Deng, Peter Gohl, Xinyue Chen, Mikael Gencay |
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| Rok vydání: | 2018 |
| Předmět: |
0301 basic medicine
China Hepatitis B virus HBsAg Genotype Population Biology medicine.disease_cause Southeast asian Hepatitis b surface antigen 03 medical and health sciences 0302 clinical medicine Asian People Virology Republic of Korea Prevalence medicine Humans education Immunoassay education.field_of_study Hepatitis B Surface Antigens medicine.diagnostic_test Genetic Variation High-Throughput Nucleotide Sequencing virus diseases Sequence Analysis DNA Hepatitis B medicine.disease digestive system diseases 030104 developmental biology Infectious Diseases Vietnam Mutant Proteins 030211 gastroenterology & hepatology |
| Zdroj: | Journal of Clinical Virology. 103:48-56 |
| ISSN: | 1386-6532 |
| Popis: | Background To avoid false negative results, hepatitis B surface antigen (HBsAg) assays need to detect samples with mutations in the immunodominant ‘a’ determinant region, which vary by ethnographic region. Objective We evaluated the prevalence and type of HBsAg mutations in a hepatitis B virus (HBV)-infected East- and Southeast Asian population, and the diagnostic performance of the Elecsys® HBsAg II Qualitative assay. Study design We analyzed 898 samples from patients with HBV infection from four sites (China [Beijing and Guangzhou], Korea and Vietnam). HBsAg mutations were detected and sequenced using highly sensitive ultra-deep sequencing and compared between the first (amino acids 124–137) and second (amino acids 139–147) loops of the ‘a’ determinant region using the Elecsys® HBsAg II Qualitative assay. Results Overall, 237 distinct amino acid mutations in the major hydrophilic region were identified; mutations were present in 660 of 898 HBV-infected patient samples (73.5%). Within the pool of 237 distinct mutations, the majority of the amino acid mutations were found in HBV genotype C (64.8%). We identified 25 previously unknown distinct mutations, mostly prevalent in genotype C-infected Korean patients (n = 18) followed by Chinese (n = 12) patients. All 898 samples were correctly identified by the Elecsys® HBsAg II Qualitative assay. Conclusions We observed 237 distinct (including 25 novel) mutations, demonstrating the complexity of HBsAg variants in HBV-infected East- and Southeast Asian patients. The Elecsys® HBsAg II Qualitative assay can reliably detect HBV-positive samples and is suitable for routine diagnostic use in East and Southeast Asia. |
| Databáze: | OpenAIRE |
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