Epidemiología molecular de un brote de infecciones por Streptococcus pyogenes en una unidad de quemados
| Autor: | Catalina Alonso M, Ana María Frola M, María Teresa Ulloa F, Soledad Prat M., Erna Cona T, Germán Ebensperger D, Andrea Galanti D, Jorge Fernández O, Alberto Fica C |
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| Jazyk: | Spanish; Castilian |
| Rok vydání: | 2003 |
| Předmět: |
Molecular epidemiology
Streptococcus pyogenes Outbreak Toxic shock syndrome General Medicine Biology medicine.disease_cause medicine.disease Group A RAPD Microbiology law.invention law Molecular sequence data medicine Pulsed-field gel electrophoresis Molecular probe technics Disease outbreaks Polymerase chain reaction |
| Zdroj: | Revista médica de Chile, Volume: 131, Issue: 2, Pages: 145-154, Published: FEB 2003 Revista médica de Chile v.131 n.2 2003 SciELO Chile CONICYT Chile instacron:CONICYT |
| Popis: | Group A Streptococcal (GAS) infections have increased in frequency and severity worldwide. During April 1996, a nosocomial outbreak associated to GAS infections affected seven patients admitted to a pediatric burn unit. The causative organism was likely disseminated from the source patient to another child in the emergency room before he was transferred to the burn unit. Patients developed burn infections or invasive disease. One of them died due to a toxic shock syndrome and 3 other lost their skin grafts. Perineal and nasal microbiological surveillance of 42 related health care workers identified only one of them as carrier of S pyogenes. Aim: To report a molecular analysis of an apparently clonal outbreak. Material and methods: The available isolates were analyzed by molecular methods including random amplified polymorphic DNA analysis (RAPD) with 4 different primers, Sma-I pulsed field gel electrophoresis (PFGE) analysis, and speA, speB and speC detection by polymerase chain reaction (PCR). Results: Two phylogenetically distant and sequentially isolated bacterial groups were identified either by RAPD analysis with selected primers or by Smal-PFGE analysis. The first group involved isolates identified in two patients that included the lethal case. The second bacterial group comprised 5 clinical isolates and the perineal and nasal isolates obtained from a health care worker. Only strains belonging to the first group harbored the speA gene and were associated with invasive disease. The second group could be split further in two subgroups according to their speB profile. Conclusions: RAPD analysis with selected primers can reproduce the PFGE-discriminating ability on the epidemiological analysis of GAS infections (Rev Med Chile 2003; 131: 145-54) |
| Databáze: | OpenAIRE |
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