Preparation of Human Immunoglobulin by Ammonium Sulfate Precipitation
| Autor: | Robert D. Francis, A. F. S. A. Habeeb |
|---|---|
| Rok vydání: | 1976 |
| Předmět: |
Ammonium sulfate
Plasmin Size-exclusion chromatography Immunoelectrophoresis Chemical Fractionation Antibodies Viral Chromatography DEAE-Cellulose chemistry.chemical_compound medicine Chemical Precipitation Humans Fibrinolysin Fragmentation (cell biology) Serum Albumin Ammonium sulfate precipitation Chromatography biology medicine.diagnostic_test Plasminogen Complement System Proteins Hematology General Medicine Electrophoresis Disc Electrophoresis chemistry Biochemistry Ammonium Sulfate Blood Preservation Immunoglobulin G biology.protein Antibody medicine.drug |
| Zdroj: | Vox Sanguinis. 31:423-434 |
| ISSN: | 1423-0410 0042-9007 |
| DOI: | 10.1159/000467349 |
| Popis: | Crude IgG precipitated from plasma by the addition of an equal volume of 4 M ammonium sulfate was fractionated on DEAE-cellulose column to obtain pure IgG as shown by disc electrophoresis, immunoelectrophoresis and gel filtration. The processed IgG was free of plasmin and plasminogen and did not undergo fragmentation or aggregation on storage at 40 degrees C for at least 4 weeks. The anticomplement activity was low and the antibody activity was retained. |
| Databáze: | OpenAIRE |
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