Identification of a novel nidovirus in an outbreak of fatal respiratory disease in ball pythons (Python regius)
Autor: | Elizabeth L. Buckles, Rafal Tokarz, Isamara Navarrete-Macias, Komal Jain, Walter Ian Lipkin, Alexandra Petrosov, Denise McAloose, Allison L. Hicks, Robert J. Ossiboff, James K. Morrisey, Lorenzo Uccellini, Ricardo de Matos |
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Jazyk: | angličtina |
Předmět: |
Male
Snake Molecular Sequence Data Respiratory Tract Diseases Short Report Reptile Nidovirales Nidovirales Infections Animal Diseases Disease Outbreaks Viral genetics Virology Boidae medicine Animals Phylogeny biology Extramural Respiratory disease Outbreak Pneumonia Sequence Analysis DNA biology.organism_classification medicine.disease 3. Good health Infectious Diseases Python (genus) RNA Viral Female Animals diseases |
Zdroj: | Virology Journal |
ISSN: | 1743-422X |
DOI: | 10.1186/1743-422x-11-144 |
Popis: | Background Respiratory infections are important causes of morbidity and mortality in reptiles; however, the causative agents are only infrequently identified. Findings Pneumonia, tracheitis and esophagitis were reported in a collection of ball pythons (Python regius). Eight of 12 snakes had evidence of bacterial pneumonia. High-throughput sequencing of total extracted nucleic acids from lung, esophagus and spleen revealed a novel nidovirus. PCR indicated the presence of viral RNA in lung, trachea, esophagus, liver, and spleen. In situ hybridization confirmed the presence of intracellular, intracytoplasmic viral nucleic acids in the lungs of infected snakes. Phylogenetic analysis based on a 1,136 amino acid segment of the polyprotein suggests that this virus may represent a new species in the subfamily Torovirinae. Conclusions This report of a novel nidovirus in ball pythons may provide insight into the pathogenesis of respiratory disease in this species and enhances our knowledge of the diversity of nidoviruses. |
Databáze: | OpenAIRE |
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