Molecular basis for hair loss in mice carrying a novel nonsense mutation (Hrrh-R ) in the hairless gene (Hr)
| Autor: | John P. Sundberg, K. T. Cain, Edward J. Michaud, Brynn H. Voy, Donald A. Carpenter, Suchita Das, Yutao Liu |
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| Rok vydání: | 2010 |
| Předmět: |
Nonsense mutation
Mutant Biology Article Exon Mice medicine Animals Alleles Oligonucleotide Array Sequence Analysis Mice Hairless General Veterinary integumentary system Gene Expression Profiling Hair follicle medicine.disease Blotting Northern Phenotype Molecular biology Hairless Gene expression profiling medicine.anatomical_structure Hair loss Genes Codon Nonsense Hair Follicle Transcription Factors |
| Zdroj: | Veterinary pathology. 47(1) |
| ISSN: | 1544-2217 |
| Popis: | Animal models carrying mutations in the hairless ( Hr) gene provide a rich resource for study of hair follicle biology. A spontaneous mouse mutant with a phenotype strikingly similar to rhino mutants of Hr arose spontaneously in the mouse facility at Oak Ridge National Laboratory. Sequence analysis of Hr in these mutants uncovered a nonsense mutation in exon 12, designated as Hrrh-R (rhino, Oak Ridge). The mutation led to significant reduction in Hr mRNA levels, predicted to be due to nonsense-mediated decay. Histological analysis indicated dilated hair follicle infundibula at 14 days of age that rapidly became filled with cornified material. Microarray analyses revealed that expression levels of many genes involved in keratinocyte differentiation, epidermal regeneration, and wound healing were significantly upregulated before morphological detection of the phenotype, suggesting their role in onset of the Hrrh-R phenotype. Identification of this new Hr allele and the underlying molecular alterations allows further understanding of the role of Hr in hair follicle biology. |
| Databáze: | OpenAIRE |
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