Variable manifestations, diverse seroreactivity and post-treatment persistence in non-human primates exposed to Borrelia burgdorferi by tick feeding
| Autor: | Amanda C. Tardo, Emir Hodzic, Mario T. Philipp, Nicole R. Hasenkampf, Lara A. Doyle-Meyers, Monica E. Embers, Mary B. Jacobs |
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| Rok vydání: | 2017 |
| Předmět: |
0301 basic medicine
Physiology lcsh:Medicine Artificial Gene Amplification and Extension Monkeys Pathology and Laboratory Medicine Polymerase Chain Reaction Biochemistry Mice Lyme disease Antibiotics Immune Physiology Medicine and Health Sciences lcsh:Science Mammals Doxycycline Immune System Proteins Multidisciplinary Spirochetes biology Antimicrobials Eukaryota Drugs Heart Bacterial Pathogens 3. Good health Medical Microbiology Vertebrates Pathogens Anatomy Antibody Macaque Research Article medicine.drug Primates Borrelia Burgdorferi Immunology 030106 microbiology Tick Research and Analysis Methods Microbiology Antibodies 03 medical and health sciences Antigen Microbial Control Old World monkeys medicine Animals Borrelia burgdorferi Molecular Biology Techniques Microbial Pathogens Molecular Biology Pharmacology Tick Bites Bacteria Ixodes business.industry Borrelia lcsh:R Organisms Biology and Life Sciences Proteins Reverse Transcriptase-Polymerase Chain Reaction medicine.disease biology.organism_classification 030104 developmental biology Amniotes Cardiovascular Anatomy biology.protein lcsh:Q business Xenodiagnosis |
| Zdroj: | PLoS ONE PLoS ONE, Vol 12, Iss 12, p e0189071 (2017) |
| ISSN: | 1932-6203 |
| DOI: | 10.1371/journal.pone.0189071 |
| Popis: | The efficacy and accepted regimen of antibiotic treatment for Lyme disease has been a point of significant contention among physicians and patients. While experimental studies in animals have offered evidence of post-treatment persistence of Borrelia burgdorferi, variations in methodology, detection methods and limitations of the models have led to some uncertainty with respect to translation of these results to human infection. With all stages of clinical Lyme disease having previously been described in nonhuman primates, this animal model was selected in order to most closely mimic human infection and response to treatment. Rhesus macaques were inoculated with B. burgdorferi by tick bite and a portion were treated with recommended doses of doxycycline for 28 days at four months post-inoculation. Signs of infection, clinical pathology, and antibody responses to a set of five antigens were monitored throughout the ~1.2 year study. Persistence of B. burgdorferi was evaluated using xenodiagnosis, bioassays in mice, multiple methods of molecular detection, immunostaining with polyclonal and monoclonal antibodies and an in vivo culture system. Our results demonstrate host-dependent signs of infection and variation in antibody responses. In addition, we observed evidence of persistent, intact, metabolically-active B. burgdorferi after antibiotic treatment of disseminated infection and showed that persistence may not be reflected by maintenance of specific antibody production by the host. |
| Databáze: | OpenAIRE |
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