Prophylactic strategies for acute hemolysis secondary to plasma-incompatible platelet transfusions: correlation between qualitative hemolysin test and isohemagglutinin titration
| Autor: | Cinthia Silvestre Landim, Francisco C. A. Gomes, Carla Luana Dinardo, Alfredo Mendrone-Junior, Bernardete Martin Zeza |
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| Rok vydání: | 2015 |
| Předmět: |
Blood group incompatibility
Red Cell ABO blood-group system lcsh:RC633-647.5 business.industry Hemolysin lcsh:Diseases of the blood and blood-forming organs Hematology Hemolysin Proteins medicine.disease Hemolysin proteins Hemolysis Isohemagglutinin Platelet transfusion ABO blood group system Immunology medicine Platelet Original Article business |
| Zdroj: | Revista Brasileira de Hematologia e Hemoterapia Revista Brasileira de Hematologia e Hemoterapia, Vol 37, Iss 4, Pp 217-222 (2015) Revista Brasileira de Hematologia e Hemoterapia v.37 n.4 2015 Revista brasileira de hematologia e hemoterapia Associação Brasileira de Hematologia e Hemoterapia e Terapia Celular (ABHHTC) instacron:ABHHTC Revista Brasileira de Hematologia e Hemoterapia, Volume: 37, Issue: 4, Pages: 217-222, Published: AUG 2015 |
| ISSN: | 1516-8484 |
| DOI: | 10.1016/j.bjhh.2015.05.001 |
| Popis: | OBJECTIVE: Brazilian legislation has recently suggested the use of the qualitative hemolysin test instead of isohemagglutinin titers as prophylaxis for acute hemolysis related to plasma-incompatible platelet transfusions. The efficacy of this test in preventing hemolytic reactions has never been evaluated while isohemagglutinin titers have been extensively studied. The main objective of this study was to evaluate the correlation between the results of these two tests. The impact of each type of prophylaxis on the platelet inventory management and the ability of the qualitative hemolysin test to prevent red cell sensitization after the transfusion of incompatible units were also studied. METHODS: A total of 246 donor blood samples were evaluated using both isohemagglutinin titers and the qualitative hemolysin test, and the results were statistically compared. Subsequently, 600 platelet units were tested using the hemolysin assay and the percentage of units unsuitable for transfusion was compared to historical data using isohemagglutinin titers (cut-off: 100). Moreover, ten patients who received units with minor ABO incompatibilities that were negative for hemolysis according to the qualitative hemolysin test were evaluated regarding the development of hemolysis and red cell sensitization (anti-A or anti-B). RESULTS: Isohemagglutinin titration and the results of qualitative hemolysin test did not correlate. The routine implementation of the qualitative hemolysin test significantly increased the percentage of platelet units found unsuitable for transfusions (15-65%; p-value |
| Databáze: | OpenAIRE |
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