Use of transcriptomic profiling to identify candidate genes involved in Polyporus umbellatus sclerotial formation affected by oxalic acid
| Autor: | Shun-Xing Guo, Xiaomei Chen, Xu Zeng, Li-Si Zhou, Yong-Mei Xing, Bing Li, Tae-Soo Lee, Min-Woong Lee |
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| Rok vydání: | 2021 |
| Předmět: |
Science
Oxalic acid Fungus medicine.disease_cause Microbiology Article Transcriptome chemistry.chemical_compound medicine Biomass Calcium Signaling Mycelium chemistry.chemical_classification Reactive oxygen species Multidisciplinary biology Polyporus umbellatus Gene Expression Profiling Oxalic Acid Hydrogen Peroxide biology.organism_classification Polyporus Oxidative Stress Gene Expression Regulation chemistry Biochemistry Medicine Calcium Efflux Energy Metabolism Reactive Oxygen Species Oxidative stress Biotechnology |
| Zdroj: | Scientific Reports, Vol 11, Iss 1, Pp 1-10 (2021) Scientific Reports |
| ISSN: | 2045-2322 |
| Popis: | Polyporus umbellatus is a precious medicinal fungus. Oxalic acid was observed to affect sclerotial formation and sclerotia possessed more medicinal compounds than mycelia. In this study, the transcriptome of P. umbellatus was analysed after the fungus was exposed to various concentrations of oxalic acid. The differentially expressed genes (DEGs) encoding a series of oxidases were upregulated, and reductases were downregulated, in the low-oxalic-acid (Low OA) group compared to the control (No OA) group, while the opposite phenomenon was observed in the high-oxalic-acid (High OA) group. The detection of reactive oxygen species (ROS) in P. umbellatus mycelia was performed visually, and Ca2+ and H2O2 fluxes were measured using non-invasive micro-test technology (NMT). The sclerotial biomass in the Low OA group increased by 66%, however, no sclerotia formed in the High OA group. The ROS fluorescence intensity increased significantly in the Low OA group but decreased considerably in the High OA group. Ca2+ and H2O2 influx significantly increased in the Low OA group, while H2O2 exhibited efflux in the High OA group. A higher level of oxidative stress formed in the Low OA group. Different concentrations of oxalic acid were determined to affect P. umbellatus sclerotial formation in different ways. |
| Databáze: | OpenAIRE |
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