Ancestral function ofInhibitors-of-kappaB regulates Caenorhabditis elegans development
| Autor: | Irene Pecharroman, Albert García-López, Lluís Campos-Vicens, David Brena, Dmytro Kukhtar, Abul B. M. M. K. Islam, Julián Cerón, Yolanda Guillén, Joan Bertran, Laura Marruecos, Lierni Fernández, Anna Bigas, Lluis Espinosa, Montserrat Porta-de-la-Riva, Eric Cornes |
|---|---|
| Rok vydání: | 2020 |
| Předmět: |
Nematodes
Cellular differentiation Mutant lcsh:Medicine Polycomb-Group Proteins Evolutionary biology Development Article Cromatina Anàlisi de proteïnes Developmental biology Genetics Animals lcsh:Science Caenorhabditis elegans Caenorhabditis elegans Proteins Metabolisme--Aspectes genètics Multidisciplinary biology Effector lcsh:R Cell Differentiation biology.organism_classification Chromatin Gene regulation Cell biology Histone biology.protein Demethylase Epigenetics lcsh:Q I-kappa B Proteins Signal transduction Analysis of proteins Genètica |
| Zdroj: | Dipòsit Digital de la UB Universidad de Barcelona Scientific Reports, Vol 10, Iss 1, Pp 1-13 (2020) Scientific Reports |
| Popis: | SummaryMammalian IκB proteins (IκBs) exert their main function as negative regulators of NF-κB, a central signaling pathway controlling immunity and inflammation. An alternative chromatin role for IκBs has been shown to affect stemness and cell differentiation. However, the involvement of NF-κB in this function has not been excluded. NFKI-1 and IKB-1 are IκB homologs inCaenorhabditis elegans, which lacks NF-κB nuclear effectors. We found thatnfki-1andikb-1mutants display developmental defects that phenocopy mutations in Polycomb and UTX-1 histone demethylase, suggesting a role forC. elegansIκBs in chromatin regulation. Further supporting this possibility(i)we detected NFKI-1 in the nucleus of cells;(ii)NFKI-1 and IKB-1 bind to histones and Polycomb proteins,(iii)and associate with chromatinin vivo, and(iv)mutations innfki-1andikb-1alter chromatin marks. Based on these results, we propose that ancestral IκB inhibitors modulate Polycomb activity at specific gene subsets with an impact on development. |
| Databáze: | OpenAIRE |
| Externí odkaz: |
|