Diagnosis and isolation of Toxoplasma gondii in horses from Brazilian slaughterhouses
| Autor: | Beatriz de Souza Lima Nino, Dauton Luiz Zulpo, Italmar Teodorico Navarro, Jonatas Campos de Almeida, Roberta Lemos Freire, Fernanda Evers, João Luis Garcia, Maria Paula de Carvalho Ewald, Sthefany Pagliari |
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| Rok vydání: | 2013 |
| Předmět: |
Prevalence
Antibodies Protozoan Toxoplasma gondii Biology law.invention law parasitic diseases medicine Animals Seroprevalence Horses Genotyping Direct fluorescent antibody horses Polymerase chain reaction nested PCR-RFLP General Veterinary indirect fluorescent antibody test medicine.disease biology.organism_classification Virology Toxoplasmosis Neospora caninum Toxoplasmosis Animal Slaughterhouse bioassay Horse Diseases Parasitology Toxoplasma Abattoirs Brazil |
| Zdroj: | Revista Brasileira de Parasitologia Veterinária v.22 n.1 2013 Revista Brasileira de Parasitologia Veterinária Colégio Brasileiro de Parasitologia Veterinária (CBPV) instacron:CBPV |
| ISSN: | 1984-2961 |
| DOI: | 10.1590/s1984-29612013005000009 |
| Popis: | This study aimed to investigate anti-Toxoplasma gondii antibodies and to isolate the parasite from the brains of horses processed at slaughterhouses in Brazil. We collected brain and blood samples from 398 horses of various ages, from six Brazilian states. Serum samples were evaluated by indirect fluorescent antibody test (IFAT cut-off titre ≥ 1:64), and brains were submitted to mouse bioassay. Among the 398 horses, positivity for T. gondii was identified in 46 (11.6%) by IFAT and in 14 (3.5%) by mouse bioassay. In 12 of those 14 bioassays, mice were positive only by IFAT (cut-off titre ≥ 1:16), T. gondii being isolated in the remaining two. Using polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of 18S rDNA to differentiate among T. gondii, Neospora caninum, and Sarcocystis neurona, we found that two of the 14 brains were positive for T. gondii only. For genotyping of the two isolates and the PCR-positive brain, we performed PCR-RFLP based on 13 markers, and SAG2 all samples were Toxoplasma gondii type I. Collectively, IFAT of horse sera and mouse bioassay identified positivity in 60 (15%) of the samples. Our results show that some horses sent to slaughter in Brazil have been exposed toT. gondii. |
| Databáze: | OpenAIRE |
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