N‐acetylcysteine prevents oxidized low‐density lipoprotein‐induced reduction of MG53 and enhances MG53 protective effect on bone marrow stem cells

Autor: Chandrakala Aluganti Narasimhulu, Yixi Li, Jia Zhang, Tao Tan, Hong Hao, Meng Jiang, Catherine M. Verfaillie, Yuqi Cui, Chunlin Yang, Zhenguo Liu, Jianjie Ma, Xin Li, Sampath Parthasarathy, Lingjuan Liu, Hua Zhu, Yuan Xiao
Rok vydání: 2019
Předmět:
Male
0301 basic medicine
Antioxidant
medicine.medical_treatment
Apoptosis
Research & Experimental Medicine
Pharmacology
Acetylcysteine
Mice
0302 clinical medicine
MEMBRANE REPAIR
N‐acetylcysteine
bone marrow stem cell
Chemistry
Cell Cycle
Free Radical Scavengers
3. Good health
Lipoproteins
LDL
medicine.anatomical_structure
Medicine
Research & Experimental
membrane damage
030220 oncology & carcinogenesis
MG53
oxidized low‐density lipoprotein
Molecular Medicine
Original Article
lipids (amino acids
peptides
and proteins)
Life Sciences & Biomedicine
medicine.drug
PROTEINS
S-NITROSYLATION
Bone Marrow Cells
LDL
03 medical and health sciences
In vivo
INJURY
medicine
Animals
Progenitor cell
PLASMA-LEVELS
Cell Proliferation
Science & Technology
oxidized low-density lipoprotein
TRANSPLANTATION
Multipotent Stem Cells
ADULT PROGENITOR CELLS
Membrane Proteins
Original Articles
Cell Biology
Protective Factors
N-acetylcysteine
MUSCULAR-DYSTROPHY
In vitro
Rats
ENDOTHELIAL DIFFERENTIATION
Mice
Inbred C57BL
030104 developmental biology
Gene Expression Regulation
multipotent adult progenitor cells
Bone marrow
Lipoprotein
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
Popis: MG53 is an important membrane repair protein and partially protects bone marrow multipotent adult progenitor cells (MAPCs) against oxidized low-density lipoprotein (ox-LDL). The present study was to test the hypothesis that the limited protective effect of MG53 on MAPCs was due to ox-LDL-induced reduction of MG53. MAPCs were cultured with and without ox-LDL (0-20 μg/mL) for up to 48 hours with or without MG53 and antioxidant N-acetylcysteine (NAC). Serum MG53 level was measured in ox-LDL-treated mice with or without NAC treatment. Ox-LDL induced significant membrane damage and substantially impaired MAPC survival with selective inhibition of Akt phosphorylation. NAC treatment effectively prevented ox-LDL-induced reduction of Akt phosphorylation without protecting MAPCs against ox-LDL. While having no effect on Akt phosphorylation, MG53 significantly decreased ox-LDL-induced membrane damage and partially improved the survival, proliferation and apoptosis of MAPCs in vitro. Ox-LDL significantly decreased MG53 level in vitro and serum MG53 level in vivo without changing MG53 clearance. NAC treatment prevented ox-LDL-induced MG53 reduction both in vitro and in vivo. Combined NAC and MG53 treatment significantly improved MAPC survival against ox-LDL. These data suggested that NAC enhanced the protective effect of MG53 on MAPCs against ox-LDL through preventing ox-LDL-induced reduction of MG53. ispartof: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE vol:24 issue:1 pages:886-898 ispartof: location:England status: published
Databáze: OpenAIRE
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