N‐acetylcysteine prevents oxidized low‐density lipoprotein‐induced reduction of MG53 and enhances MG53 protective effect on bone marrow stem cells
Autor: | Chandrakala Aluganti Narasimhulu, Yixi Li, Jia Zhang, Tao Tan, Hong Hao, Meng Jiang, Catherine M. Verfaillie, Yuqi Cui, Chunlin Yang, Zhenguo Liu, Jianjie Ma, Xin Li, Sampath Parthasarathy, Lingjuan Liu, Hua Zhu, Yuan Xiao |
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Rok vydání: | 2019 |
Předmět: |
Male
0301 basic medicine Antioxidant medicine.medical_treatment Apoptosis Research & Experimental Medicine Pharmacology Acetylcysteine Mice 0302 clinical medicine MEMBRANE REPAIR N‐acetylcysteine bone marrow stem cell Chemistry Cell Cycle Free Radical Scavengers 3. Good health Lipoproteins LDL medicine.anatomical_structure Medicine Research & Experimental membrane damage 030220 oncology & carcinogenesis MG53 oxidized low‐density lipoprotein Molecular Medicine Original Article lipids (amino acids peptides and proteins) Life Sciences & Biomedicine medicine.drug PROTEINS S-NITROSYLATION Bone Marrow Cells LDL 03 medical and health sciences In vivo INJURY medicine Animals Progenitor cell PLASMA-LEVELS Cell Proliferation Science & Technology oxidized low-density lipoprotein TRANSPLANTATION Multipotent Stem Cells ADULT PROGENITOR CELLS Membrane Proteins Original Articles Cell Biology Protective Factors N-acetylcysteine MUSCULAR-DYSTROPHY In vitro Rats ENDOTHELIAL DIFFERENTIATION Mice Inbred C57BL 030104 developmental biology Gene Expression Regulation multipotent adult progenitor cells Bone marrow Lipoprotein |
Zdroj: | Journal of Cellular and Molecular Medicine |
ISSN: | 1582-4934 1582-1838 |
Popis: | MG53 is an important membrane repair protein and partially protects bone marrow multipotent adult progenitor cells (MAPCs) against oxidized low-density lipoprotein (ox-LDL). The present study was to test the hypothesis that the limited protective effect of MG53 on MAPCs was due to ox-LDL-induced reduction of MG53. MAPCs were cultured with and without ox-LDL (0-20 μg/mL) for up to 48 hours with or without MG53 and antioxidant N-acetylcysteine (NAC). Serum MG53 level was measured in ox-LDL-treated mice with or without NAC treatment. Ox-LDL induced significant membrane damage and substantially impaired MAPC survival with selective inhibition of Akt phosphorylation. NAC treatment effectively prevented ox-LDL-induced reduction of Akt phosphorylation without protecting MAPCs against ox-LDL. While having no effect on Akt phosphorylation, MG53 significantly decreased ox-LDL-induced membrane damage and partially improved the survival, proliferation and apoptosis of MAPCs in vitro. Ox-LDL significantly decreased MG53 level in vitro and serum MG53 level in vivo without changing MG53 clearance. NAC treatment prevented ox-LDL-induced MG53 reduction both in vitro and in vivo. Combined NAC and MG53 treatment significantly improved MAPC survival against ox-LDL. These data suggested that NAC enhanced the protective effect of MG53 on MAPCs against ox-LDL through preventing ox-LDL-induced reduction of MG53. ispartof: JOURNAL OF CELLULAR AND MOLECULAR MEDICINE vol:24 issue:1 pages:886-898 ispartof: location:England status: published |
Databáze: | OpenAIRE |
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