Flow-Cytometric investigation of Cellular Metabolism during Oxidative Stress and the Effect of Tocopherol
| Autor: | G Valet, C Reinke, H Leuenberger, U Moser, S Amann |
|---|---|
| Rok vydání: | 1999 |
| Předmět: |
Antioxidant
Endocrinology Diabetes and Metabolism medicine.medical_treatment Intracellular pH Medicine (miscellaneous) Cell Separation medicine.disease_cause Antioxidants Membrane Potentials chemistry.chemical_compound medicine Humans Vitamin E Tocopherol Viability assay Cells Cultured Skin Nutrition and Dietetics General Medicine Glutathione Fibroblasts Hydrogen-Ion Concentration Flow Cytometry Oxidative Stress chemistry Biochemistry Intracellular Oxidative stress |
| Zdroj: | International Journal for Vitamin and Nutrition Research. :0356-0361 |
| ISSN: | 0300-9831 |
| DOI: | 10.1024/0300-9831.69.5.356 |
| Popis: | Many studies and scientific publications report on potentially beneficial effects of the lipophilic anti-oxidant vitamin E on cellular metabolic pathways. The present work presents data on the influence of tocopherol on different intracellular parameters of intact and living human skin fibroblasts by flow-cytometric measurements. The parameters analysed were the intracellular pH, representing cell metabolism and cell function, intracellular glutathione, representing one of the cell's own radical scavenger enzyme systems, membrane potential and cell viability. In order to cause large numbers of free radicals cells were UVB-irradiated prior to measurement. The results of the flow-cytometric measurements indicate that vitamin E has significant protecting effects on the measured biochemical parameters during oxidative stress. In the presence of the lipophilic radical scavenger a significant stabilizing effect on pH, intracellular glutathione levels and membrane potential could be observed. Furthermore, vitamin E administration was associated with increased cell viability after UVB irradiation. |
| Databáze: | OpenAIRE |
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