Simple HPLC Method for Quantitative Determination of Carbinoxamine in Human Plasma: Application in Pharmacokinetic Studies and Therapeutic Drug Monitoring
| Autor: | Rasha M. El-Morsi, Soha M. El-Masry |
|---|---|
| Rok vydání: | 2019 |
| Předmět: |
Male
Adolescent Pyridines Cost-Benefit Analysis Liquid-Liquid Extraction Ethyl acetate Administration Oral Sensitivity and Specificity 01 natural sciences High-performance liquid chromatography Young Adult 03 medical and health sciences chemistry.chemical_compound 0302 clinical medicine Pharmacokinetics Limit of Detection Drug Discovery medicine Humans Acetonitrile Phosphoric acid Chromatography High Pressure Liquid Chromatography medicine.diagnostic_test 010405 organic chemistry Extraction (chemistry) Reproducibility of Results General Medicine Healthy Volunteers 0104 chemical sciences chemistry Therapeutic drug monitoring 030220 oncology & carcinogenesis Histamine H1 Antagonists Feasibility Studies Carbinoxamine Spectrophotometry Ultraviolet Drug Monitoring medicine.drug |
| Zdroj: | Drug Research. 69:342-347 |
| ISSN: | 2194-9387 2194-9379 |
| Popis: | A sensitive, specific and cost-effective HPLC method was developed for quantitative determination of carbinoxamine in human plasma using liquid chromatography with ultraviolet detection. A simple liquid–liquid extraction by ethyl acetate was used to extract carbinoxamine from human plasma. Satisfactory separation was achieved by a mobile phase consisting of 20 mM ammonium dihydrogen orthophosphate containing 0.01% triethyl amine (pH adjusted to 3 by using phosphoric acid) and acetonitrile in ratio of (75:25, v/v) at a flow rate of 0.9 mL/min on Hypersil® BDS C18 column (250×4.6 mm, 5 μm) column. The UV detector was set at 260 nm. The developed method was validated in human plasma with a lower limit of quantitation of 5 ng/mL for carbinoxamine. Linearity was demonstrated over the concentration range 5–200 ng/mL. The observed within- and between-day assay precision ranged from 1.902 to 14.90%; accuracy varied between 98.87 and 108.0%. This method can be used suitably for pharmacokinetic studies and in therapeutic drug monitoring in patients treated with carbinoxamine. |
| Databáze: | OpenAIRE |
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