LyP‐1‐fMWNTs enhanced targeted delivery of MBD1siRNA to pancreatic cancer cells

Autor: Deliang Fu, Ya Gao, Zhi-Bo Xie, Lie Yao, Yifan Zhang, Quan‐Jun Lin
Rok vydání: 2020
Předmět:
active targeting
0301 basic medicine
Cell Survival
Genetic enhancement
pancreatic cancer
Mice
Nude
Apoptosis
Peptide
Carbon nanotube
Adenocarcinoma
Transfection
Peptides
Cyclic
law.invention
03 medical and health sciences
RNA interference
0302 clinical medicine
law
Cell Line
Tumor
Pancreatic cancer
medicine
Zeta potential
Animals
Humans
RNA
Small Interfering
Cell Proliferation
chemistry.chemical_classification
LyP‐l peptide
Mice
Inbred BALB C
Cell Death
Nanotubes
Carbon
multi‐walled carbon nanotubes
Original Articles
Cell Biology
medicine.disease
Endocytosis
In vitro
DNA-Binding Proteins
Gene Expression Regulation
Neoplastic
Pancreatic Neoplasms
030104 developmental biology
chemistry
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
Original Article
Carcinoma
Pancreatic Ductal
Transcription Factors
Zdroj: Journal of Cellular and Molecular Medicine
ISSN: 1582-4934
1582-1838
DOI: 10.1111/jcmm.14864
Popis: Functionalized multi‐walled carbon nanotubes have been extensively gained popularity in pancreatic cancer gene therapy. LyP‐1, a peptide, has been proved to specifically bind pancreatic cancer cells. The potential therapeutic effect of LyP‐1–conjugated functionalized multi‐walled carbon nanotubes in treating pancreatic cancer is still unknown. In this study, LyP‐1–conjugated functionalized multi‐walled carbon nanotubes were successfully synthesized, characterized and showed satisfactory size distribution and zeta potential. Compared with functionalized multi‐walled carbon nanotubes, cellular uptake of LyP‐1–functionalized multi‐walled carbon nanotubes was shown to be increased. Compound of LyP‐1–functionalized multi‐walled carbon nanotubes and MBD1siRNA showed superior gene transfection efficiency. Moreover, LyP‐1‐fMWNTs/MBD1siRNA complex could significantly decrease the viability and proliferation and promoted apoptosis of pancreatic cancer cells in vitro. Further xenograft assays revealed that the tumour burden in the nude mice injected with LyP‐1–functionalized multi‐walled carbon nanotubes/MBD1siRNA was significantly relieved. The study demonstrated that LyP‐1–functionalized multi‐walled carbon nanotubes/MBD1siRNA could be a promising candidate for tumour active targeting therapy in pancreatic cancer.
Databáze: OpenAIRE
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