The epidermal growth factor ortholog of ectromelia virus activates EGFR/ErbB1 and demonstrates mitogenic function in vitro
| Autor: | Julie M. Schrey, Rosie M. Zimmerman, Kaylyn Haan, Rebecca A. Morgis, Adam R. Hersperger |
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| Rok vydání: | 2021 |
| Předmět: |
Glycosylation
Ectromelia virus medicine.medical_treatment Vaccinia virus Cell Line S Phase Ectromelia Mice Viral Proteins Cell Movement Epidermal growth factor Virology medicine Animals Humans Doubling time Receptor Wound Healing Epidermal Growth Factor biology Growth factor biology.organism_classification medicine.disease In vitro Cell biology ErbB Receptors Transmembrane domain Intercellular Signaling Peptides and Proteins Mitogens Cell Division Protein Binding Signal Transduction |
| Zdroj: | Virology. 564:1-12 |
| ISSN: | 0042-6822 |
| Popis: | Many poxviruses produce proteins that are related to epidermal growth factor (EGF). Prior genome sequencing of ectromelia virus revealed a gene predicted to produce a protein with homology to EGF, which we refer to as ectromelia growth factor (ECGF). ECGF is truncated relative to vaccinia growth factor (VGF) because the former lacks a transmembrane domain. We show these proteins can experience differential N-linked glycosylation. Despite these differences, both proteins maintain the six conserved cysteine residues important for the function of EGF. Since ECGF has not been characterized, our objective was to determine if it can act as a growth factor. We added ECGF to cultured cells and found that the EGF receptor becomes activated, S-phase was induced, doubling time decreased, and in vitro wound healing occurred faster compared to untreated cells. In summary, we demonstrate that ECGF can act as a mitogen in a similar manner as VGF. |
| Databáze: | OpenAIRE |
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